Comparative Cr, As and CCA induced Cytostaticity in mice kidney: A contribution to assess CCA toxicity

• Published in: Environmental toxicology and pharmacology Vol. 73; p. 103297
• Main Authors: Matos, Rita Cerejeira, Oliveira, Helena, Fonseca, Henrique M.A.C., Morais, Simone, Sharma, Bechan, Santos, Conceição, de Lourdes Pereira, Maria
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2020; Elsevier Science Ltd
• Subjects: Arsenates; Arsenates - toxicity; Arsenic; Arsenic - toxicity; Arsenicals; Arsenite; CCA; Cell cycle; Chromium; Chromium - toxicity; Chromium Compounds; Chromium oxides; Chromium trioxide; Clastogenicity; Copper arsenate; Copper chrome arsenate; Cytostatic Agents - toxicity; Cytostaticity; Durability; Flow cytometry; G1 phase; Hazardous Substances - toxicity; Health hazards; Kidney; Kidneys; Leaching; Oxides - toxicity; Parks & recreation areas; Playgrounds; Preservatives; Public health; Statistical analysis; Statistical methods; Toxicity; Toxicity Tests; Waste management; Waste streams; Wood; Wood products; CCA; Chromium; Clastogenicity; Cytostaticity; Arsenite; Kidney
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2019.103297

•CCA has toxic effects on the cell cycle dynamics of mouse kidneys, distinct from its compounds.•CCA and As compounds showed clastogenic effects, particularly in the S phase.•Cell cycle dynamics is a valuable endpoint to assess the putative toxicity of remaining CCA-treated infrastructures.•Genotoxicity of CCA and its constituents in renal cells claims for their ecofriendly removal prior leakage.
CCA (Chromium Copper Arsenate) treated wood, widely used in outdoor residential structures and playgrounds, poses considerable dangers of leaching of its components to the environment.
In this study, mouse kidney samples were used to evaluate the effects of CCA, chromium trioxide (CrO3) and arsenic pentoxide (As2O5) on cell pathophysiology by flow cytometry. Samples were collected after 14, 24, 48 and 96 h of animal exposure. While Cr had no statistically significant cytostatic effects, As2O5 induced a S-phase delay in animals exposed for 24 h, and over time a G0/G1 phase blockage. The effects of CCA in S-phase were similar, but more severe than those of As2O5. Since environmental and public health hazards due to the long durability of CCA-treated wood products, these data confirm that CCA has profoundly toxic effects on cell cycle, distinct from the compounds themselves. These cytostatic effects support cell cycle dynamics as a valuable endpoint to assess the toxicity of remaining CCA-treated infrastructures, and the expected increased waste stream over the coming decades.