WT1 isoform expression pattern in acute myeloid leukemia

• Published in: Leukemia research Vol. 37; no. 12; pp. 1744 - 1749
• Main Authors: Luna, Irene, Such, Esperanza, Cervera, Jose, Barragán, Eva, Ibañez, Mariam, Gómez-Seguí, Inés, López-Pavía, María, Llop, Marta, Fuster, Oscar, Dolz, Sandra, Oltra, Silvestre, Alonso, Carmen, Vera, Belén, Lorenzo, Ignacio, Martínez-Cuadrón, David, Montesinos, Pau, Senent, M. Leonor, Moscardó, Federico, Bolufer, Pascual, Sanz, Miguel A
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2013
• Subjects: Acute myeloid leukemia; Adolescent; Adult; Aged; Aged, 80 and over; Case-Control Studies; Cohort Studies; Female; Gene Expression Profiling; Gene Expression Regulation, Leukemic; Hematology, Oncology and Palliative Medicine; HL-60 Cells; Humans; K562 Cells; Leukemia, Myeloid, Acute - genetics; Male; Middle Aged; Protein Isoforms - genetics; Tumor Cells, Cultured; WT1 isoforms; WT1 Proteins - genetics; Young Adult; Acute myeloid leukemia; WT1 isoforms
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2013.10.009

Abstract WT1 plays a dual role in leukemia development, probably due to an imbalance in the expression of the 4 main WT1 isoforms. We quantify their expression and evaluate them in a series of AML patients. Our data showed a predominant expression of isoform D in AML, although in a lower quantity than in normal CD34+ cells. We found a positive correlation between the total WT1 expression and A, B and C isoforms. The overexpression of WT1 in AML might be due to a relative increase in A, B and C isoforms, together with a relative decrease in isoform D expression.