Anti-inflammatory effects of pravastatin on Helicobacter Pylori-induced gastritis in mice

• Published in: Digestive diseases and sciences Vol. 52; no. 10; pp. 2833 - 2839
• Main Authors: YAMATO, Masanori, WATANABE, Toshio, ARAKAWA, Tetsuo, HIGUCHI, Kazuhide, TAIRA, Koichi, TANIGAWA, Tetsuya, SHIBA, Masatsugu, TOMINAGA, Kazunari, FUJIWARA, Yasuhiro, OSHITANI, Nobuhide, TAKEUCHI, Koji
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-10-2007; Springer Nature B.V
• Subjects: Animals; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Biological and medical sciences; Biomarkers - metabolism; Disease Models, Animal; Female; Follow-Up Studies; Gastric Mucosa - metabolism; Gastric Mucosa - microbiology; Gastric Mucosa - pathology; Gastritis - drug therapy; Gastritis - etiology; Gastritis - pathology; Gene Expression - drug effects; Helicobacter; Helicobacter Infections - complications; Helicobacter Infections - drug therapy; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - isolation & purification; Helicobacter pylori - pathogenicity; Human bacterial diseases; Hydroxymethylglutaryl-CoA Reductase Inhibitors - therapeutic use; Immunohistochemistry; Infectious diseases; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Medical sciences; Mice; Mice, Inbred C57BL; Nitric Oxide Synthase Type III - genetics; Nitric Oxide Synthase Type III - metabolism; Pravastatin - therapeutic use; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Treatment Outcome; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Spirillaceae; H. pylori; Pravastatin; Helicobacter pylori; Bacteria; Gastric disease; Spirillales; Cytokines; Enzyme; Rodentia; Cytokine; Enzyme inhibitor; Statin derivative; Statin; Nitric-oxide synthase; Gastritis; Vertebrata; Endothelial nitric oxide synthase; Mammalia; Mouse; Animal; Digestive diseases; Hydroxymethylglutaryl-CoA reductase; Oxidoreductases; Antilipemic agent
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-006-9638-7

Pravastatin, an HMG-CoA reductase inhibitor, exerts anti-inflammatory effects via several mechanisms including induction of endothelial nitric oxide synthase (eNOS). We investigated the effect of pravastatin on Helicobacter pylori-induced gastritis in mice. Mice with or without H. pylori infection received intraperitoneal pravastatin daily for 1 week. Expression of eNOS mRNA and tumor necrosis factor-alpha mRNA and myeloperoxidase activity in gastric tissue was determined. Myeloperoxidase activity was reduced in a dose-dependent manner by pravastatin, with activity inhibited by 53.5 and 73.7% at doses of 0.3 and 1 mg/kg, respectively. At a dose of 1 mg/kg, pravastatin reduced the level of tumor necrosis factor-alpha mRNA by 52.7%, while it did not affect eNOS expression. Pravastatin had no effects on these inflammatory parameters in uninfected mice. Pravastatin did not affect the viability of H. pylori. In conclusion, pravastatin exerts an anti-inflammatory effect on H. pylori-induced gastritis in mice without affecting eNOS expression.