HPA-1, 3, 5 genotyping to establish a typed platelet donor panel

• Published in: Pathology Vol. 32; no. 2; pp. 89 - 93
• Main Authors: Verran, J., Grey, D., Bennett, J., Lown, J.A.G., Erber, W.N.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01-05-2000; Informa UK Ltd; Taylor and Francis
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antigens, Human Platelet - genetics; Antigens, Human Platelet - immunology; Biological and medical sciences; Blood Donors; Blood Grouping and Crossmatching - methods; Blood Platelets - immunology; Blood Transfusion; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; DNA - analysis; Donor panel; Epitopes - genetics; Genotype; genotyping; HPA; human platelet antigens; Humans; Medical sciences; PCR-RFLP; PCR-SSP; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Registries; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Western Australia; Western Australia; Donor panel; PCR-SSP; genotyping; HPA; NAIT; PCR-RFLP; PTP; human platelet antigens; Antigen; Human; Polymerase chain reaction; Platelet; Transfusion; Typing; Genotype; Molecular biology; Blood donor
• ISSN: 0031-3025; 1465-3931
• DOI: 10.1080/003130200104295

Platelet antigen typing of blood donors is not routinely performed. Usually, ABO-matched platelets are transfused. In situations where the recipient has a human platelet-associated (HPA) antibody resulting in refractoriness to platelet transfusions, post-transfusion purpura or neonatal alloimmune thrombocytopenia, HPA-typed platelets may be required. Having a panel of HPA-typed donors would be helpful. This study utilised PCR-RFLP and PCR-SSP methods to determine the HPA-1, 3, 5 genotypes of 1,000 blood donors to establish a panel of HPA homozygous donors who could donate platelets for patients with anti-HPA-antibodies. The study demonstrates the HPA genotype frequencies in a Western Australian blood donor population and has identified donors who are homozygous for the “aa” and “bb” genotypes for HPA-1, 3 and 5. The Australian Red Cross Blood Service-NW Region can now use these data to develop a panel of HPA-1, 3 and 5 “aa” and “bb” homozygous platelet donors, which can be accessed for patients with HPA antibodies requiring platelet transfusion.