NMR studies of DNA duplexes singly cross-linked by different synthetic linkers

• Published in: Nucleic acids research Vol. 23; no. 23; pp. 4827 - 4835
• Main Authors: Altmann, Serge, Labhardt, Alexander M., Bur, Daniel, Lehmann, Christain, Bannwarth, Willi, Billeter, Martin, Wüthrich, Kurt, Leupin, Werner
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 11-12-1995
• Subjects: Base Sequence; Carbohydrate Conformation; Carbohydrate Sequence; DNA - chemistry; DNA - ultrastructure; Drug Design; Magnetic Resonance Spectroscopy; Models, Chemical; Molecular Sequence Data; Nucleic Acid Heteroduplexes - chemistry; Nucleic Acid Heteroduplexes - ultrastructure
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/23.23.4827

Molecular modelling studies resulted in the design of a variety of non-nucleotidic covalent linkers to bridge the 3′-end of the (+)-strand and the 5′-end of the (−)-strand in DNA duplexes. Three of these linkers were synthesized and used to prepare singly cross-linked duplexes d(GTGGAATTC)–linker–d(GAATTCCAC). Linker I is an assembly of a propylene-, a phosphateand a second propylene-group and is thought to mimic the backbone of two nucleotides. Linker II and III consist of five and six ethyleneglycol units, respectively. The melting temperatures of the cross-linked duplexes are 65°C for I and 73°C for II and III, as compared with 36°C for the corresponding non-linked nonadeoxynucleotide duplex. The three cross-linked duplexes were structurally characterized by nuclear magnetic resonance spectroscopy. The 1H and 31P resonance assignments in the DNA stem were obtained using standard methods. For the resonance assignment of the linker protons, two-dimensional 1H-31P heteronuclear COSY and two-quantumexperiments were used. Distance geometry calculations with NOE-derived distance constraints were performed and the resulting structures were energyminimized. In duplex I, the nucleotides flanking the propylene-phosphate-propylene-linker do not form a Watson–Crick base pair, whereas in duplexes II and III the entire DNA stem is in a B-type double helix conformation.