Ultrasound as an alternative technology to extract carotenoids and lipids from Heterochlorella luteoviridis

• Published in: Bioresource technology Vol. 224; pp. 753 - 757
• Main Authors: Jaeschke, Débora Pez, Rech, Rosane, Marczak, Ligia Damasceno Ferreira, Mercali, Giovana Domeneghini
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-01-2017
• Subjects: Carotenoids - isolation & purification; Chemical Fractionation - methods; Chlorophyta - chemistry; Ethanol; Ethanol - chemistry; Fatty acids; Lipids - isolation & purification; Microalgae; Microalgae - chemistry; Pigments; Ultrasonics - methods; Pigments; Microalgae; Ethanol; Fatty acids
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.11.107

•An alternative carotenoid and lipid extraction method from microalgae was studied.•Ultrasound was evaluated as a pre-treatment to the extraction.•Ethanol at different concentrations was evaluated as extraction solvent.•The methodology showed to be attractive for carotenoid extraction.
The present work evaluated the use of ultrasound as a pre-treatment to lipid and carotenoid extraction from the microalgae Heterochlorella luteoviridis. The pre-treatment was performed in the presence of ethanol (25%, v/v) with the ultrasound intensity varying from 0 to 100% (435kJkg−1). After the pre-treatment, a diffusive step was performed in order to evaluate different ethanol concentrations (50–75%, v/v). The results regarding carotenoid extraction showed that there is an optimal extraction region: 40–80% of ultrasound intensity and 60–75% of ethanol concentration. The lipid extraction was not influenced by the ultrasound pre-treatment and increased with the increase of ethanol concentration.