Composition and cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica)

• Published in: Carbohydrate polymers Vol. 89; no. 4; pp. 1022 - 1026
• Main Authors: Peng, Zhenfei, Liu, Min, Fang, Zhexiang, Wu, Jiulin, Zhang, Qiqing
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-08-2012; Elsevier
• Subjects: Antineoplastic agents; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antitumor activity; Antitumor agents; Applied sciences; Biological and medical sciences; Carbohydrate Conformation; Carbohydrates; Cell Line, Tumor; Chemotherapy; Cytotoxicity; Cytotoxins - chemistry; Cytotoxins - isolation & purification; Cytotoxins - pharmacology; dose response; Exact sciences and technology; fucose; Humans; Laminaria - chemistry; Laminaria japonica; Laminaria japonica polysaccharide; Low cytotoxicity; Mannose; Medical sciences; Molecular weight; monosaccharides; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - pathology; myocytes; Myocytes, Smooth Muscle - metabolism; Myocytes, Smooth Muscle - pathology; Natural polymers; Neoplasms - drug therapy; Neoplasms - metabolism; Neoplasms - pathology; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Rhamnose; Saccharina japonica; Smooth muscle; Starch and polysaccharides; Structure characterization; Toxicity; Laminaria japonica polysaccharide; Antitumor activity; Structure characterization; Low cytotoxicity; Antineoplastic agent; Algae; Chemical isolation; Experimental study; In vitro; Biological activity; Water soluble compound; Concentration effect; Plant origin; Oside polymer; Tumor cell
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.03.043

► A water-soluble polysaccharide WPS-2-1 was purified from Laminaria japonica. ► WPS-2-1 was composed of mannose, rhamnose and fucose. ► WPS-2-1 had a backbone of array by (1→4)-glycosidic linkages. ► WPS-2-1 exhibited higher antitumor activities and lower cytotoxicity on VSMCs. ► WPS-2-1 may be considered as a potential candidate for antitumor agent.
A novel polysaccharide WPS-2-1, with an average molecular weight of 80kDa, was purified from aqueous extracts of Laminaria japonica. Monosaccharides analysis revealed that WPS-2-1 was composed of mannose, rhamnose and fucose with a molar ratio of 1.0:2.3:1.2. Analysis by periodate oxidation-Smith degradation indicated that WPS-2-1 had a backbone of array by (1→4)-glycosidic linkages. Cytotoxicity assay showed that WPS-2-1 presented significantly higher antitumor activities against A375 and BGC823 cells with a dose-dependent manner, and exhibited lower cytotoxicity to vascular smooth muscle cells. The results suggested that WPS-2-1 should be explored as a potential antitumor agent with low toxicity.