Intragraft B cell differentiation during the development of tolerance to kidney allografts is associated with a regulatory B cell signature revealed by single cell transcriptomics

• Published in: American journal of transplantation Vol. 23; no. 9; pp. 1319 - 1330
• Main Authors: Guinn, Michael Tyler, Szuter, Edward S., Yokose, Takahiro, Ge, Jifu, Rosales, Ivy A., Chetal, Kashish, Sadreyev, Ruslan I., Cuenca, Alex G., Kreisel, Daniel, Sage, Peter T., Russell, Paul S., Madsen, Joren C., Colvin, Robert B., Alessandrini, Alessandro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-09-2023
• Subjects: Allografts; Animals; B cell biology; B-Lymphocytes, Regulatory; Cell Differentiation; Graft Rejection - etiology; Graft Survival; immunogenetics; informatics; Kidney; kidney transplantation; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; molecular biology; tolerance; Transcriptome; molecular biology; rTLOs; B cell biology; kidney transplantation; immunogenetics; Breg; C57BL/6; DT; FITC; scRNA-seq; FSC; Tfh; tolerance; TIM-1; Acc; UMAP; Tfr; SSC; TOLS; Treg; informatics; DBA/2J; MST; BCR; PE; Rej; Ig
• ISSN: 1600-6135; 1600-6143; 1600-6143
• DOI: 10.1016/j.ajt.2023.05.036

Mouse kidney allografts are spontaneously accepted in select, fully mismatched donor-recipient strain combinations, like DBA/2J to C57BL/6 (B6), by natural tolerance. We previously showed accepted renal grafts form aggregates containing various immune cells within 2 weeks posttransplant, referred to as regulatory T cell–rich organized lymphoid structures, which are a novel regulatory tertiary lymphoid organ. To characterize the cells within T cell–rich organized lymphoid structures, we performed single-cell RNA sequencing on CD45+ sorted cells from accepted and rejected renal grafts from 1-week to 6-months posttransplant. Analysis of single-cell RNA sequencing data revealed a shifting from a T cell–dominant to a B cell–rich population by 6 months with an increased regulatory B cell signature. Furthermore, B cells were a greater proportion of the early infiltrating cells in accepted vs rejecting grafts. Flow cytometry of B cells at 20 weeks posttransplant revealed T cell, immunoglobulin domain and mucin domain-1+ B cells, potentially implicating a regulatory role in the maintenance of allograft tolerance. Lastly, B cell trajectory analysis revealed intragraft differentiation from precursor B cells to memory B cells in accepted allografts. In summary, we show a shifting T cell– to B cell–rich environment and a differential cellular pattern among accepted vs rejecting kidney allografts, possibly implicating B cells in the maintenance of kidney allograft acceptance.
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