Infectious Disease Agents Associated with Pulmonary Alterations in Aborted Bovine Fetuses

• Published in: Animals (Basel) Vol. 12; no. 13; p. 1596
• Main Authors: de Oliveira, Thalita Evani Silva, Scuisato, Gabriela Sanches, Fritzen, Juliana Torres Tomazi, Silva, Denise Correia, Massi, Rodrigo Pelisson, Pelaquim, Isadora Fernanda, Silva, Luara Evangelista, Flores, Eduardo Furtado, Lima Santos, Renato, Pretto-Giordano, Lucienne Garcia, Lisbôa, Júlio Augusto Naylor, Alfieri, Amauri Alcindo, Headley, Selwyn Arlington
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 21-06-2022; MDPI
• Subjects: abortion; Animal tissues; Antigens; Assaying; Bacteria; BoGHV6; Bronchopneumonia; BRSV; Brucella abortus; BVDV; Cattle; Deoxyribonucleic acid; Diarrhea; Disease; DNA; Evaluation; fetopathy; Fetuses; Gram-positive bacteria; Histopathology; Infections; Infectious diseases; interstitial pneumonia; Laboratories; Leptospira; Lung diseases; Lungs; Mortality; Mycoplasma bovis; Neospora caninum; Nucleic acids; Parainfluenza; Pathogens; Pneumonia; Reproductive pathology; Respiratory diseases; Respiratory syncytial virus; Software; Viruses; Brazil; United States > US
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani12131596

This study investigated the occurrence of selected pathogens of bovine respiratory disease in fetal pulmonary tissue of cattle and associated these with patterns of disease. Fetal pulmonary (n = 37) tissues were evaluated by histopathology; immunohistochemical assays identified intralesional antigens of bovine alphaherpesvirus 1 (BoAHV1), bovine viral diarrhea virus (BVDV), bovine parainfluenza virus 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), and Mycoplasma bovis. Molecular assays were performed to amplify reproductive disease pathogens and bovine gammaherpesvirus 6 (BoGHV6) from 12 lungs. The 2 patterns of pulmonary diseases were interstitial pneumonia (12/37) and suppurative bronchopneumonia (1/37). The frequency of the intralesional antigens identified was BRSV (16.2%; 6/37), BVDV (13.5%; 5/37), BoAHV1 (8.1%; 3/37), M. bovis (5.4%; 2/37), and BPIV-3 (2.7%; 1/37). Interstitial pneumonia was associated with BRSV (n = 3), BoAHV1 (n = 3), and BVDV (n = 2); suppurative bronchopneumonia contained a Gram-positive bacterium and BVDV and BRSV. Reproductive pathogens detected included Leptospira spp., (n = 3), BVDV, Neospora caninum, and Brucella abortus (n = 2). BoGHV6 DNA was identified in the lungs of two fetuses with interstitial pneumonia. These findings suggest that these fetuses were infected transplacentally by several pathogens. The role of some of these pathogens herein identified must be further elucidated in the possible participation of fetal disease.