Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages

RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides were well tolerated in the passenger strand of siR...

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Bibliographic Details
Published in:	Organic & biomolecular chemistry Vol. 12; no. 8; p. 1207
Main Authors:	Tanui, Paul, Kennedy, Scott D, Lunstad, Benjamin D, Haas, Amanda, Leake, Devin, Rozners, Eriks
Format:	Journal Article
Language:	English
Published:	England 28-02-2014
Subjects:	Amides - chemistry Base Sequence HeLa Cells Humans Models, Molecular Nucleic Acid Conformation RNA Interference RNA, Small Interfering - administration & dosage RNA, Small Interfering - chemical synthesis RNA, Small Interfering - chemistry RNA, Small Interfering - genetics
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Summary:	RNA sequences having up to three consecutive internal amide linkages were synthesized and studied using UV and NMR spectroscopy. The amide modifications did not interfere with normal base-pairing and A-type RNA conformation. Three consecutive amides were well tolerated in the passenger strand of siRNA and caused little change in RNAi activity.
ISSN:	1477-0539
DOI:	10.1039/c3ob42532k