The use of 12-hydroxyheptadecatrienoic acid (HHT) as an HPLC/spectrophotometric marker for cyclooxygenase pathway activity in resident rat peritoneal cells
12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes...
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| Published in: | Prostaglandins leukotrienes and medicine Vol. 26; no. 3; p. 171 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
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Scotland
01-03-1987
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| Abstract | 12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes in a single system. |
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| AbstractList | 12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes in a single system. |
| Author | Eskra, J D Sweeney, F J Carty, T J Pereira, M J |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/3033686$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_plefa_2004_11_002 crossref_primary_10_1007_s12630_022_02378_y crossref_primary_10_1054_plef_2000_0228 crossref_primary_10_1007_s00216_007_1718_9 crossref_primary_10_1016_j_bja_2022_05_005 crossref_primary_10_1016_0167_4889_89_90015_3 crossref_primary_10_1111_j_2042_7158_1992_tb03636_x crossref_primary_10_1016_0006_2952_89_90069_5 crossref_primary_10_1194_jlr_M037754 crossref_primary_10_1007_BF02265116 |
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| Snippet | 12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat... |
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| SubjectTerms | Animals Arachidonate 5-Lipoxygenase - analysis Arachidonic Acids - metabolism Ascitic Fluid - cytology Calcimycin - pharmacology Chromatography, High Pressure Liquid - methods Cyclooxygenase Inhibitors Eosinophils - drug effects Eosinophils - enzymology Fatty Acids, Unsaturated Hydroxyeicosatetraenoic Acids - analysis Hydroxyeicosatetraenoic Acids - biosynthesis Leukotriene B4 - analysis Leukotriene B4 - biosynthesis Lipoxygenase Inhibitors Macrophages - drug effects Macrophages - enzymology Male Mast Cells - drug effects Mast Cells - enzymology Prostaglandin Antagonists - pharmacology Prostaglandin-Endoperoxide Synthases - analysis Rats Rats, Inbred Strains Reference Standards Spectrophotometry, Ultraviolet - methods |
| Title | The use of 12-hydroxyheptadecatrienoic acid (HHT) as an HPLC/spectrophotometric marker for cyclooxygenase pathway activity in resident rat peritoneal cells |
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