The use of 12-hydroxyheptadecatrienoic acid (HHT) as an HPLC/spectrophotometric marker for cyclooxygenase pathway activity in resident rat peritoneal cells

12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes...

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Bibliographic Details
Published in:	Prostaglandins leukotrienes and medicine Vol. 26; no. 3; p. 171
Main Authors:	Sweeney, F J, Pereira, M J, Eskra, J D, Carty, T J
Format:	Journal Article
Language:	English
Published:	Scotland 01-03-1987
Subjects:	Animals Arachidonate 5-Lipoxygenase - analysis Arachidonic Acids - metabolism Ascitic Fluid - cytology Calcimycin - pharmacology Chromatography, High Pressure Liquid - methods Cyclooxygenase Inhibitors Eosinophils - drug effects Eosinophils - enzymology Fatty Acids, Unsaturated Hydroxyeicosatetraenoic Acids - analysis Hydroxyeicosatetraenoic Acids - biosynthesis Leukotriene B4 - analysis Leukotriene B4 - biosynthesis Lipoxygenase Inhibitors Macrophages - drug effects Macrophages - enzymology Male Mast Cells - drug effects Mast Cells - enzymology Prostaglandin Antagonists - pharmacology Prostaglandin-Endoperoxide Synthases - analysis Rats Rats, Inbred Strains Reference Standards Spectrophotometry, Ultraviolet - methods
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Summary:	12-Hydroxyheptadecatrienoic acid (HHT), a UV chromophore, has been used to assess cyclooxygenase (CO) pathway metabolism of arachidonic acid (AA) by rat peritoneal cells. Simultaneous monitoring at 235 and 280 nm after HPLC permits the measurement of both CO and 5-lipoxygenase (5-LO) pathway fluxes in a single system.
ISSN:	0262-1746
DOI:	10.1016/0262-1746(87)90027-8