Anti-inflammatory effects of the protein hydrolysate and peptide fractions isolated from Salvia hispanica L. seeds

• Published in: Food and agricultural immunology Vol. 30; no. 1; pp. 786 - 803
• Main Authors: Chan-Zapata, Ivan, Arana-Argáez, Víctor Ermilo, Torres-Romero, Julio Cesar, Segura-Campos, Maira Rubí
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01-01-2019; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Animal models; Anti-inflammatory agents; Cell activation; Cytokines; Derivatives; Dinitrofluorobenzene; Edema; Hydrogen peroxide; Hypersensitivity; Hypersensitivity (delayed); Inflammation; Interleukin 1; Interleukin 10; Interleukin 6; Macrophages; Membranes; Mucilage; Peptides; Peritoneum; Proteins; Salvia hispanica; Salvia hispanica L; Seeds; Tumor necrosis factor-α; Ultrafiltration
• ISSN: 0954-0105; 1465-3443
• DOI: 10.1080/09540105.2019.1632804

The aim of this work was to evaluate the potential anti-inflammatory effect of protein hydrolysate and peptide fractions from Salvia hispanica L. seeds. Protein isolate was obtained using defatted flour of mucilage-free seeds. Hydrolysis process was conducted by enzymatic digestion. The hydrolysate was fractionated using ultrafiltration membranes to obtain the peptide fractions (<1, 1-3, 3-5, 5-10, and >10 kDa). Protein derivatives were evaluated by in vitro activation of murine peritoneal macrophages. The anti-inflammatory activity was determined as NO production, H
2
O
2
release and pro- and anti-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-10) production. All the peptides exerted an anti-inflammatory activity, but peptide fraction between 1 - 3 kDa showed the highest anti-inflammatory effect. This fraction was evaluated on in vivo murine models of TPA-induced ear edema and DNFB-induced delayed-type hypersensitivity, exhibiting inhibitory effects. Hence, the results demonstrated that protein derivatives from S. hispanica L. seeds have in vitro and in vivo anti-inflammatory effects.