Recovery of Indigenous probiotic Lactobacillus plantarum Mut-7 on healthy Indonesian adults after consumption of fermented milk containing these bacteria

• Published in: Journal of food science and technology Vol. 58; no. 9; pp. 3525 - 3532
• Main Authors: Harahap, I. A., Mariyatun, M., Hasan, P. N., Pamungkaningtyas, F. H., Widada, J., Utami, T., Cahyanto, M. N., Juffrie, M., Dinoto, A., Nurfiani, S., Zulaichah, E., Sujaya, I. N., Rahayu, E. S.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-09-2021; Springer Nature B.V
• Subjects: Adults; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Consumption; Deoxyribonucleic acid; DNA; DNA sequencing; E coli; Feces; Fermented milk products; Food Science; Gastrointestinal system; Gastrointestinal tract; Indigenous plants; Ingestion; Intestinal microflora; Lactobacilli; Lactobacillus plantarum; Microbiota; Microorganisms; Milk; Nutrition; Original; Original Article; Polymerase chain reaction; Probiotics; Recovery; rRNA 16S; Starter cultures; Indigenous probiotic; Fermented milk; Feces
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-021-05046-z

Probiotics are live microorganisms that confer health benefits on the host when administered in adequate amounts, such as to support the balance of gut microbiota. In this study, the selected indigenous probiotic strain,
Lactobacillus plantarum
Mut-7, was used as a starter culture to produce fermented milk. A total of 28 healthy Indonesian youngsters and adults (male/female: 16/12; age 19.4–22.9 years old; normal BMI range 18.6–22.7 kg/m
2
) were supplemented with the fermented milk. This study aimed to determine the recovery of
L. plantarum
Mut-7 through molecular analysis from the subjects feces after ingestion of 140 mL fermented milk containing 7.0 log
10
CFU/ml of
L. plantarum
Mut-7 for 15 days. Molecular detection was performed using the rep-PCR technique and sequencing of DNA 16S rRNA. Consumption of fermented milk containing
L. plantarum
Mut-7 enabled reduction of total
E. coli
and
Coliform
non-
E. coli
in several subjects. It was able to increase the total LAB and total
L. plantarum
in subjects’ feces. The number of
L. plantarum
and mesophilic LAB increased by 5.5 ± 1.6 log
10
CFU/g, 1.8 ± 0.8 log
10
. On the other side, thermophilic LAB increased by 2.8 ± 3.0 log
10
CFU/g in 23 out of 28 subjects. These findings proved that
L. plantarum
survived in the human gastrointestinal tract. Based on the molecular identification technique using rep-PCR technique and sequencing of gene 16S rRNA, two isolates had similarity to
L. plantarum
Mut-7 by a coefficient value of 100%.