Localization of vascular endothelial growth factor during the early reparative phase of the rats' vessels deprivation-induced osteonecrosis of the femoral heads

• Published in: Experimental and molecular pathology Vol. 77; no. 2; pp. 145 - 148
• Main Authors: Vadasz, Z., Misselevich, I., Norman, D., Peled, E., H. Boss, J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-10-2004; Elsevier
• Subjects: Angiogenesis; Animal model of human disease; Animals; Biological and medical sciences; Bone Marrow - pathology; Diseases of the osteoarticular system; Experimental osteonecrosis of the femoral heads of rats; Femoral capital osteonecrosis; Femur Head - blood supply; Femur Head - pathology; Fibroblasts - immunology; Fibroblasts - metabolism; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Necrosis; Osteonecrosis - pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Perthes disease; Rats; Rats, Sprague-Dawley; Regional Blood Flow; Synovial Membrane - blood supply; Synovial Membrane - metabolism; Synovial Membrane - pathology; Vascular bone diseases; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Angiogenesis; Femoral capital osteonecrosis; Perthes disease; Vascular endothelial growth factor; Animal model of human disease; Experimental osteonecrosis of the femoral heads of rats; Animal model; Femur; Disease; Rat; Femoral head; Diseases of the osteoarticular system; Osteonecrosis; Blood vessel; Osteochondritis; Neovascularization; Localization; Human; Early phase; Rodentia; Experimental study; Hip; Vertebrata; Anatomic pathology; Mammalia; Vascular endothelium growth factor; Animal; Circulatory system
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2004.06.002

The expedited revascularization of the rats' avascular, necrotic femoral heads suggests the operation of angiogenic factor(s). The blood circulation of the epiphysis was interrupted by cutting the cervical periosteum and the ligamentum teres of rats' femoral heads. Three days postoperatively, the marrow was necrotic. Seven days postoperatively, the subchondral bony plate and trabecular bone were necrotic as well. The joint capsule was distended by myriad, so-called synovial fibroblasts, all of which were virtually immunoreactive with an antibody to vascular endothelial growth factor. The expression of this factor in the synovial membrane of non-operated rats was limited to preexisting blood vessels. Revascularization of necrotic, avascular femoral heads makes up the essential step in the chain of events terminating in the repair processes, that is, resorption of the necrotic debris and its substitution by newly formed bony and hematopoietic-fatty tissues. Synthesis and release of excessive amounts of vascular endothelial growth factor by these fibroblasts explain the lively angiogenesis in the necrotic intertrabecular spaces of the femoral heads.