Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells

• Published in: Journal of structural biology Vol. 201; no. 1; pp. 15 - 25
• Main Authors: Carter, Stephen D., Mageswaran, Shrawan K., Farino, Zachary J., Mamede, João I., Oikonomou, Catherine M., Hope, Thomas J., Freyberg, Zachary, Jensen, Grant J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-2018
• Subjects: Autofluorescence; Cryo-CLEM; Electron cryo-tomography; Fluorescent proteins; Mammalian cells; Cryo-CLEM; Autofluorescence; Fluorescent proteins; Electron cryo-tomography; Mammalian cells
• ISSN: 1047-8477; 1095-8657
• DOI: 10.1016/j.jsb.2017.10.009

In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit strong punctate autofluorescence when imaged under cryogenic conditions (80 K). Autofluorescence originated from multilamellar bodies (MLBs) and secretory granules. Here we describe a method to distinguish fluorescent protein tags from these autofluorescent sources based on the narrower emission spectrum of the former. The method is first tested on mitochondria and then applied to examine the ultrastructural variability of secretory granules within insulin-secreting pancreatic beta-cell-derived INS-1E cells.