Elevated Concentration of Adenosine 3′:5′-cyclic Monophosphate in Intestinal Mucosa after Treatment with Cholera Toxin

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 67; no. 2; pp. 851 - 856
• Main Authors: Schafer, David E., Lust, W. David, Sircar, Bithi, Goldberg, Nelson D.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-10-1970; National Acad Sciences
• Subjects: Animals; Biological Sciences: Physiology; Body tissues; Cholera; Cholera - metabolism; Control loops; Cyclic AMP - analysis; Cyclic AMP - metabolism; Dogs; Enterotoxins; Enterotoxins - pharmacology; Freezing; Intestinal mucosa; Intestinal Mucosa - analysis; Intestinal Mucosa - metabolism; Mucosa; Physiology; Secretion; Tissue samples; Toxins; Vibrio - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.67.2.851

A heat-labile factor in cell-free filtrate of a Vibrio cholerae culture induces a marked rise in the wet-weight concentration of adenosine 3′:5′-cyclic monophosphate (cyclic AMP) in the intestinal mucosa of the dog. The increase becomes appreciable 1-1.5 hr after intraluminal administration of the filtrate, about the same time as the onset of intestinal secretion in response to a heat-labile enterotoxin in the filtrate. These results are consistent with the hypothesis that cyclic AMP may be an intermediary in the intestinal secretory response to cholera toxin.