Critical analysis of application of generalized distance function for optimization of important variables for esterase synthesis by Saccharomyces cerevisiae

• Published in: Bioresource technology Vol. 99; no. 13; pp. 5545 - 5555
• Main Authors: Panda, T., Gowrishankar, B.S.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-09-2008; [New York, NY]: Elsevier Ltd; Elsevier Science
• Subjects: Biological and medical sciences; Culture Media; Esterase; Esterases - biosynthesis; Fundamental and applied biological sciences. Psychology; Genearlized distance function approach; Kinetics; Optimization; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins - biosynthesis; Single and multi-response; Substrate Specificity; Single and multi-response; Esterase; Genearlized distance function approach; Saccharomyces cerevisiae; Optimization; Ascomycota; Fungi; Enzyme; Hydrolases; Esterases
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2007.10.057

The generalized distance function approach is employed to obtain a suitable near optimal conditions of variables. The optimal values of variables (medium constituents, microbiological parameters, and process parameters) have been evaluated separately using single responses (either specific esterase activity or cell mass) as per central-composite-design and multi-responses following generalized distance function approach. The optimal conditions (medium composition (g
l
−1): dextrose, 13.43; peptone, 7.285; yeast extract, 2.55; and malt extract, 1.695; microbiological parameters: slant age, 39.9
h; inoculum age, 9.6
h; and number of cells, 1.49
×
10
8
numbers
ml
−1; process conditions: temperature, 29.9
°C; and pH, 6.2) obtained by generalized distance approach can be considered as a ‘near optimal’ solution of interactive multi-response systems of intracellular esterase synthesis by
Saccharomyces cerevisiae.