Acute and sublethal effects of two insecticides on earthworms (Lumbricus terrestris L.) under laboratory conditions

• Published in: Environmental toxicology Vol. 18; no. 1; pp. 1 - 8
• Main Authors: Mosleh, Yahia Y., Paris-Palacios, Séverine, Couderchet, Michel, Vernet, Guy
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 2003; Wiley
• Subjects: aldicarb; Aldicarb - pharmacokinetics; Aldicarb - toxicity; Animal, plant and microbial ecology; Animals; annelids; Applied ecology; Biological and medical sciences; Biomarkers - analysis; Dose-Response Relationship, Drug; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; endosulfan; Endosulfan - toxicity; Fundamental and applied biological sciences. Psychology; growth rate; Insecticides - pharmacokinetics; Insecticides - toxicity; Lethal Dose 50; Oligochaeta - growth & development; Oligochaeta - physiology; Pesticide Residues; protein content; Proteins - analysis; residue; soil; Soil Pollutants - pharmacokinetics; Soil Pollutants - toxicity; Terrestrial environment, soil, air; Time Factors; Tissue Distribution; Growth inhibitor; Insecticide; Growth rate; Toxicity; annelids; Pesticides; Biological marker; Exposure; soil; Soil pollution; Annelida; Oligochaeta; residue; Lethal dose; Organochlorine compounds; Aldicarb; endosulfan; Concentration effect; Invertebrata; Biological accumulation; Carbamate; Comparative study; Protein content; Lumbricus terrestris
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.10095

Earthworms (Lumbricus terrestris L.) were exposed to commercial formulations of endosulfan and aldicarb for 2, 7, and 15 days, and the LC10, LC25, and LC50 were determined. Worms were then exposed to LC10, LC25, and LC50 concentrations of endosulfan and LC10 and LC25 concentrations of aldicarb. The growth rate and total protein content were determined and related to endosulfan and aldicarb residues in soil and earthworms. Aldicarb was more toxic than endosulfan under the experimental conditions. The residues of endosulfan and aldicarb caused a significant reduction in the growth rate and total protein content of earthworms. The residues of endosulfan and aldicarb were monitored in soil and earthworms after 2, 7, and 15 days of exposure. The residues remaining in the soil after the experiments ranged between 37.75% and 68.54% of the applied concentration for endosulfan and between 10.13% and 67.71% of the applied concentration for aldicarb. Small amounts of both insecticides were detected in worms, and accumulation was more important for endosulfan. This study proposes the use of growth rate and total protein content as biomarkers for contamination by endosulfan and aldicarb. © 2003 Wiley Periodicals, Inc. Environ Toxicol 18: 1–8, 2003.