Arabidopsis lipoxygenase 2 is essential for formation of green leaf volatiles and five‐carbon volatiles

• Published in: FEBS letters Vol. 590; no. 7; pp. 1017 - 1027
• Main Authors: Mochizuki, Satoshi, Sugimoto, Koichi, Koeduka, Takao, Matsui, Kenji
• Format: Journal Article
• Language: English
• Published: England 01-04-2016
• Subjects: Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Catalytic Domain; Chromatography, High Pressure Liquid; Cold Temperature - adverse effects; Cyclopentanes - chemistry; Cyclopentanes - metabolism; Enzyme Activation; Gas Chromatography-Mass Spectrometry; green leaf volatiles; Isoenzymes - genetics; Isoenzymes - metabolism; jasmonate; Kinetics; lipoxygenase; Lipoxygenase - genetics; Lipoxygenase - metabolism; Lipoxygenases - genetics; Lipoxygenases - metabolism; Molecular Structure; Mutagenesis, Insertional; Mutation; Oxylipins - chemistry; Oxylipins - metabolism; Phylogeny; Plant Leaves - enzymology; Plant Leaves - metabolism; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Point Mutation; Solid Phase Microextraction; Volatile Organic Compounds - chemistry; Volatile Organic Compounds - metabolism; green leaf volatiles; lipoxygenase; Arabidopsis; jasmonate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1002/1873-3468.12133

Plants biosynthesize a variety of bioactive lipid derivatives, such as green leaf volatiles (GLVs) and jasmonates (JAs). Here we identify a lipoxygenase 2 (LOX2) involved in GLV biosynthesis in Arabidopsis using mutant lines for each of the six LOX isoforms present in Arabidopsis. We found that formation of five carbon volatiles was also dependent on LOX2. LOX2 is known to be involved in formation of JA; thus, LOX2 is apparently versatile in function. The results in this study suggested that LOX2 activity is suppressed in intact cells but activated upon tissue damage to support the rapid GLV‐burst observed in wounded leaves.