MosaicExplorerJ: Interactive stitching of terabyte-size tiled datasets from lightsheet microscopy [version 2; peer review: 2 approved]
We introduce MosaicExplorerJ, an ImageJ macro to stitch 3D tiles from terabyte-size microscopy datasets organized on a regular 2D grid. As opposed to existing software, stitching does not require any prior information on the actual positions of the tiles, or conversion of raw TIFF images to a multi-...
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Published in: | F1000 research Vol. 9; p. 1308 |
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Main Authors: | , , , , , |
Format: | Journal Article |
Language: | English |
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2021
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Abstract | We introduce MosaicExplorerJ, an ImageJ macro to stitch 3D tiles from terabyte-size microscopy datasets organized on a regular 2D grid. As opposed to existing software, stitching does not require any prior information on the actual positions of the tiles, or conversion of raw TIFF images to a multi-resolution format for interactive exploration and fast processing. MosaicExplorerJ was specifically designed to process lightsheet microscopy datasets from optically cleared samples. It can handle multiple fluorescence channels, dual-sided lightsheet illumination and dual-sided camera detection. |
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AbstractList | We introduce MosaicExplorerJ, an ImageJ macro to stitch 3D tiles from terabyte-size microscopy datasets organized on a regular 2D grid. As opposed to existing software, stitching does not require any prior information on the actual positions of the tiles, or conversion of raw TIFF images to a multi-resolution format for interactive exploration and fast processing. MosaicExplorerJ was specifically designed to process lightsheet microscopy datasets from optically cleared samples. It can handle multiple fluorescence channels, dual-sided lightsheet illumination and dual-sided camera detection. |
Author | Colombelli, Julien Bardia, Lídia Tosi, Sébastien Barallobre, Maria Jose Soto-Montenegro, María Luisa Muñoz-Barrutia, Arrate |
Author_xml | – sequence: 1 givenname: Sébastien orcidid: 0000-0001-8348-2778 surname: Tosi fullname: Tosi, Sébastien email: sebastien.tosi@irbbarcelona.org organization: Institute for Research in Biomedicine - IRB Barcelona, the Barcelona Institute for Science and Technology - BIST, Barcelona, Spain – sequence: 2 givenname: Lídia surname: Bardia fullname: Bardia, Lídia organization: Institute for Research in Biomedicine - IRB Barcelona, the Barcelona Institute for Science and Technology - BIST, Barcelona, Spain – sequence: 3 givenname: Maria Jose surname: Barallobre fullname: Barallobre, Maria Jose organization: Department of Developmental Biology, Instituto de Biología Molecular de Barcelona, CSIC, Barcelona, Spain – sequence: 4 givenname: Arrate surname: Muñoz-Barrutia fullname: Muñoz-Barrutia, Arrate organization: Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain – sequence: 5 givenname: María Luisa surname: Soto-Montenegro fullname: Soto-Montenegro, María Luisa organization: CIBER de Salud Mental (CIBERSAM), Madrid, Spain – sequence: 6 givenname: Julien orcidid: 0000-0002-2784-4276 surname: Colombelli fullname: Colombelli, Julien organization: Institute for Research in Biomedicine - IRB Barcelona, the Barcelona Institute for Science and Technology - BIST, Barcelona, Spain |
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Cites_doi | 10.1038/s41592-019-0501-0 10.1186/1471-2105-13-316 10.1111/j.1365-2818.2008.03094.x 10.1126/science.1100035 |
ContentType | Journal Article |
Copyright | Copyright: © 2021 Tosi S et al. Copyright: © 2021 Tosi S et al. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright: © 2021 Tosi S et al. 2021 |
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Keywords | ImageJ Lightsheet microscopy Tiled scan Mosaic 3D stitching |
Language | English |
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References | A Bria (ref-3) 2012; 13 S Tosi (ref-6) 2021 S Tosi (ref-7) 2021 M Emmenlauer (ref-1) 2009; 233 ref-2 J Huisken (ref-5) 2004; 305 D Hörl (ref-4) 2019; 16 |
References_xml | – volume: 16 start-page: 870-874 year: 2019 ident: ref-4 article-title: BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples. publication-title: Nat Methods. doi: 10.1038/s41592-019-0501-0 contributor: fullname: D Hörl – ident: ref-2 article-title: https://imagej.net/Grid/Collection_Stitching_Plugin – volume: 13 start-page: 316 year: 2012 ident: ref-3 article-title: TeraStitcher - a tool for fast automatic 3D-stitching of teravoxel-sized microscopy images. publication-title: BMC Bioinformatics. doi: 10.1186/1471-2105-13-316 contributor: fullname: A Bria – year: 2021 ident: ref-7 article-title: SebastienTs/MosaicExplorerJ: Revised Release (Version 1.5). publication-title: Zenodo. contributor: fullname: S Tosi – year: 2021 ident: ref-6 article-title: MosaicExplorerJ F1000Research article extended data (Version 2.0). publication-title: Zenodo. contributor: fullname: S Tosi – volume: 233 start-page: 42-60 year: 2009 ident: ref-1 article-title: XuvTools: free, fast and reliable stitching of large 3D datasets. publication-title: J Microsc. doi: 10.1111/j.1365-2818.2008.03094.x contributor: fullname: M Emmenlauer – volume: 305 start-page: 1007-1009 year: 2004 ident: ref-5 article-title: Optical sectioning deep inside live embryos by selective plane illumination microscopy. publication-title: Science. doi: 10.1126/science.1100035 contributor: fullname: J Huisken |
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Title | MosaicExplorerJ: Interactive stitching of terabyte-size tiled datasets from lightsheet microscopy [version 2; peer review: 2 approved] |
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