Determination of tobramycin in soil by HPLC with ultrasonic-assisted extraction and solid-phase extraction

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 879; no. 13; pp. 901 - 907
• Main Authors: He, Shun, Chen, Qiyou, Sun, Yan, Zhu, Yuncong, Luo, Laixin, Li, Jianqiang, Cao, Yongsong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-04-2011; Elsevier
• Subjects: acetone; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cartridges; Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Reverse-Phase; correlation; derivatization; detection limit; Drug Stability; drugs; Extraction; Fluorobenzenes - chemistry; Fundamental and applied biological sciences. Psychology; General pharmacology; high performance liquid chromatography; Humans; Hydrogen-Ion Concentration; Linear Models; Mathematical analysis; Medical sciences; methanol; Methyl alcohol; monitoring; Nitro Compounds - chemistry; Oscillations; Pharmacology. Drug treatments; Pre-column derivatization; purification methods; quantitative analysis; Reproducibility of Results; Residues; Sensitivity and Specificity; Soil; Soil (material); Soil - chemistry; Soil Pollutants - analysis; soil types; Solid Phase Extraction - methods; Solid-phase extraction; Sonication; Temperature; Tobramycin; Tobramycin - analysis; Tobramycin - blood; Tobramycin - urine; Ultrasonic-assisted extraction; ultrasonics; Soil; Ultrasonic-assisted extraction; Tobramycin; Solid-phase extraction; Pre-column derivatization; Solid phase extraction; Precolumn; HPLC chromatography; Determination; Derivatization; Chemical enrichment; Soils; Antibiotic; Ultrasound assisted extraction; Sample preparation; Aminoglycoside; Protein synthesis inhibitor; Antibacterial agent; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2011.02.042

Pharmaceuticals residues in the environment have become a growing scientific interest worldwide. In the light of the possible harmful effects of tobramycin, a rapid and sensitive analytical method for determination of tobramycin in soil was developed. The extraction and purification methods, derivatization conditions, and chromatographic conditions in the determination of tobramycin in soil have been fully investigated. Extraction was carried out by a combination of vortex mixer and ultrasonic oscillation using acetone/water as the extraction agent. The extract was concentrated to 1
mL and passed through the C
18 SPE cartridge rinsed with water (3
mL), methanol (3
mL). The derivatization procedure was followed by the reaction of tobramycin with 4-Chloro-3,5-dinitrobenzotrifluoride at 60
°C for 10
min in pH 9.0 H
3BO
3–Na
2B
4O
7 medium. The labeled tobramycin was determined by high performance liquid chromatography at 245
nm. Separation was accomplished within 15
min in gradient elution mode with trifluoroacetic acid in mobile phase as ion-pair reagent. The correlation coefficient for the method was 0.9999 in concentrations ranging from 0.10 to 100.0
μg/g. The limit of detection was 0.02
μg/g for tobramycin in soil at a signal-to-noise ratio of 3. The calculated recoveries of the proposed method were from 78.0 to 91.0% and RSDs were 3.38–9.79% in the application to the quantitative determination of tobramycin in all types of soil. The method will help to establish adequate monitoring of tobramycin residue in soil and make the contribution to environmental behavior evaluation.