Stimulation of Structural and Functional Recovery of the Kidney in Rats with Postischemic Acute Renal Failure of Different Severity by Embryonic Protein-Peptide Complex Therapy

• Published in: Bulletin of experimental biology and medicine Vol. 169; no. 5; pp. 623 - 629
• Main Authors: Kirpatovskii, V. I., Sivkov, A. V., Golovanov, S. A., Drozhzheva, V. V., Efremov, G. D., Samoilova, S. I.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2020; Springer; Springer Nature B.V
• Subjects: Acute renal failure; Biomedical and Life Sciences; Biomedicine; Body weight; Calcium; Cell Biology; Creatinine; Embryos; Epithelium; Hypertrophy; Internal Medicine; Ischemia; Kidneys; Laboratory Medicine; Molecular weight; Nephrectomy; Pathology; Peptides; Reabsorption; Recovery of function; Renal failure; Renal tubules; Structure-function relationships; Urea; embryonic tissues; acute renal failure; therapy; regenerative medicine
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04941-3

In male rats, acute renal failure was simulated by clamping the vascular pedicle of the left kidney for 60 or 90 min and right-sided nephrectomy. In the control series, no therapy was performed. In the experimental series, the animals were daily injected subcutaneously with Cellex, a protein-peptide complex (PPC) chromatographically isolated from the brain tissue of pig embryos with a molecular weight of its components from 10 to 250 kDa. PPC was administered 5 times a week (10 injections) in a dose of 0.1 ml/kg (0.1 mg active substance per 1 kg body weight). Ischemia of a single kidney led to the development of acute renal failure, more severe after 90-min ischemia. PPC therapy reduced the severity of functional disorders mainly at the early stages (3 and 7 days) with normalization of blood concentrations of urea and creatinine, creatinine clearance, tubular reabsorption of sodium and calcium, including the cases with 90-min ischemia, which did not occur in the control series. PPC therapy also contributed to hypertrophy of many glomeruli, prevented the development of glomerulosclerosis, and reduced damage to the epithelium of the renal tubules. At the same time, neither pronounced lymphohistiocytic infiltration, nor focal nephrosclerosis typical of control series were observed.