Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH stretching vib...

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Bibliographic Details
Published in:	Journal of Biomedical Optics Vol. 16; no. 2; p. 021104
Main Authors:	Lin, Chia-Yu, Potma, Eric O, Suhalim, Jeffrey L, Nien, Chyong Ly, Jester, James V, Miljkovic, Milo D, Diem, Max
Format:	Journal Article
Language:	English
Published:	United States Society of Photo-Optical Instrumentation Engineers (SPIE) 01-02-2011
Subjects:	Animals Cars Ducts Image Enhancement - methods Image Interpretation, Computer-Assisted - methods Imaging Lipids Meibomian Glands - cytology Mice Microscopy - methods Principal Component Analysis Raman scattering Reproducibility of Results Sensitivity and Specificity Special Section on Coherent Raman Imaging Techniques and Biomedical Applications Spectra Spectrum Analysis, Raman - methods Tomography, Optical Coherence - methods Utilities
Online Access:	Get full text
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