Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands
The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH stretching vib...
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| Published in: | Journal of Biomedical Optics Vol. 16; no. 2; p. 021104 |
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| Abstract | The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH
stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. |
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| AbstractList | The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH(2) stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH 2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. |
| Author | Nien, Chyong Ly Lin, Chia-Yu Diem, Max Suhalim, Jeffrey L Potma, Eric O Miljkovic, Milo D Jester, James V |
| Author_xml | – sequence: 1 givenname: Chia-Yu surname: Lin fullname: Lin, Chia-Yu organization: Univ. of California, Irvine (United States) – sequence: 2 givenname: Eric O surname: Potma fullname: Potma, Eric O organization: Univ. of California, Irvine (United States) – sequence: 3 givenname: Jeffrey L surname: Suhalim fullname: Suhalim, Jeffrey L organization: Univ. of California, Irvine (United States) – sequence: 4 givenname: Chyong Ly surname: Nien fullname: Nien, Chyong Ly organization: Univ. of California, Irvine (United States) – sequence: 5 givenname: James V surname: Jester fullname: Jester, James V organization: Univ. of California, Irvine (United States) – sequence: 6 givenname: Milo D surname: Miljkovic fullname: Miljkovic, Milo D organization: Northeastern Univ. (United States) – sequence: 7 givenname: Max surname: Diem fullname: Diem, Max organization: Northeastern Univ. (United States) |
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COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only. Copyright © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Address all correspondence to: Eric Potma, Department of Chemistry, University of California, Irvine, Irvine, CA 92697. Tel: 949-824-9942; Fax: 949-824-8571; E-mail: epotma@uci.edu. |
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| Snippet | The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets... |
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| SubjectTerms | Animals Cars Ducts Image Enhancement - methods Image Interpretation, Computer-Assisted - methods Imaging Lipids Meibomian Glands - cytology Mice Microscopy - methods Principal Component Analysis Raman scattering Reproducibility of Results Sensitivity and Specificity Special Section on Coherent Raman Imaging Techniques and Biomedical Applications Spectra Spectrum Analysis, Raman - methods Tomography, Optical Coherence - methods Utilities |
| Title | Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands |
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