Similarity Analysis of Klebsiella pneumoniae Producing Carbapenemases Isolated from UTI and Other Infections

• Published in: Antibiotics (Basel) Vol. 12; no. 7; p. 1224
• Main Authors: Pruss, Agata, Kwiatkowski, Paweł, Sienkiewicz, Monika, Masiuk, Helena, Łapińska, Agnieszka, Kot, Barbara, Kilczewska, Zuzanna, Giedrys-Kalemba, Stefania, Dołęgowska, Barbara
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24-07-2023; MDPI
• Subjects: Amides; Aminoglycosides; Antibiotics; Asymptomatic; Bacteria; Bacterial infections; Carbapenemase; carbapenemases; Cephalosporins; Drug resistance; Enzymes; Epidemics; epidemiological investigation; Genotype & phenotype; Genotypes; Health facilities; Hospitalization; Hospitals; infections; Klebsiella; Klebsiella pneumoniae; Metallography; Microorganisms; Nosocomial infections; Opportunist infection; Ostomy; Pathogens; PFGE; Phylogenetics; Phylogeny; Plasmids; Pneumonia; Public health; Quinolones; Strains (organisms); Urinary tract; Urine; β Lactamase; β-Lactam antibiotics; infections; PFGE; carbapenemases; epidemiological investigation; Klebsiella pneumoniae
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics12071224

is an important opportunistic pathogen responsible for severe infections, mainly urinary tract infections (UTIs) and pneumonia. Hospital epidemic infections caused by multiresistant strains of carbapenemase-producing
are the most concerning. NDM-producing strains are resistant to a wide range of antibiotics and have become the most significant threat. Determining the natural reservoirs and routes of infections is essential to end hospital outbreaks. Understanding the relatedness of
strains is essential to determine the range and nature of the infection. The study compared phylogenetic relatedness between multiresistant
strains isolated from hospitalized patients. Susceptibility to drugs and mechanisms of resistance were confirmed using phenotypic methods. PFGE was used to analyze the relatedness between strains. We analyzed 69
strains from various healthcare units. The isolates were mainly identified from urine. Strains were resistant to β-lactam antibiotics with β-lactamase inhibitors, cephalosporins, and quinolones. Their susceptibility to aminoglycosides and carbapenem antibiotics was diverse. Most of the isolated strains produced New Delhi metallo-ß-lactamase (NDM). Although
strains were classified into several genotype clusters, closely related isolates were confirmed in the same hospital's wards, and in two hospitals in the same province.