Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent

• Published in: International journal of molecular sciences Vol. 21; no. 4; p. 1403
• Main Authors: Szymczak, Mateusz, Grygorcewicz, Bartłomiej, Karczewska-Golec, Joanna, Decewicz, Przemysław, Pankowski, Jarosław Adam, Országh-Szturo, Hanna, Bącal, Paweł, Dołęgowska, Barbara, Golec, Piotr
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 19-02-2020; MDPI AG
• Subjects: animal model; Animals; antibiotic resistance; atrophic rhinitis; biofilm; Biofilms; Bordetella bronchiseptica - pathogenicity; Bordetella bronchiseptica - ultrastructure; Bordetella bronchiseptica - virology; Bordetella Infections - therapy; Bordetella Infections - veterinary; emerging diseases; galleria mellonella; Host Microbial Interactions; Hydrogen-Ion Concentration; Larva - microbiology; Lepidoptera - microbiology; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; phage stability; phage therapy; Phylogeny; Podoviridae - genetics; Podoviridae - growth & development; Podoviridae - radiation effects; Podoviridae - ultrastructure; Temperature; veterinary microbiology; Virion - isolation & purification; Virion - ultrastructure; zoonosis; atrophic rhinitis; emerging diseases; veterinary microbiology; antibiotic resistance; biofilm; phage stability; phage therapy; Galleria mellonella; animal model; zoonosis
• ISSN: 1422-0067; 1422-0067
• DOI: 10.3390/ijms21041403

, an emerging zoonotic pathogen, infects a broad range of mammalian hosts.
-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by
is unknown, but it has been postulated that some hypervirulent
isolates may be responsible for undiagnosed respiratory infections in humans.
was shown to acquire antibiotic resistance genes from other bacterial genera, especially
. Here, we present a new
lytic bacteriophage-vB_BbrP_BB8-of the
family, which offers a safe alternative to antibiotic treatment of
infections. We explored the phage at the level of genome, physiology, morphology, and infection kinetics. Its therapeutic potential was investigated in biofilms and in an
model, both of which mimic the natural environment of infection. The BB8 is a unique phage with a genome structure resembling that of T7-like phages. Its latent period is 75 ± 5 min and its burst size is 88 ± 10 phages. The BB8 infection causes complete lysis of
cultures irrespective of the MOI used. The phage efficiently removes bacterial biofilm and prevents the lethality induced by
in
honeycomb moth larvae.