Addition of BMP-2 or BMP-6 to dexamethasone, ascorbic acid, and β-glycerophosphate may not enhance osteogenic differentiation of human periodontal ligament cells

• Published in: Growth factors (Chur, Switzerland) Vol. 28; no. 6; pp. 437 - 446
• Main Authors: Khanna-Jain, Rashi, Agata, Hideki, Vuorinen, Annukka, Sándor, George K. B., Suuronen, Riitta, Miettinen, Susanna
• Format: Journal Article
• Language: English
• Published: England Informa Healthcare 01-12-2010; Taylor & Francis
• Subjects: Alkaline Phosphatase - analysis; Anthraquinones - analysis; ascorbic acid; Ascorbic Acid - pharmacology; Bone Morphogenetic Protein 2 - pharmacology; Bone Morphogenetic Protein 6 - pharmacology; bone morphogenetic protein-2 and -6; Calcification, Physiologic - drug effects; Calcium - analysis; Cells, Cultured; Collagen Type I - genetics; Core Binding Factor Alpha 2 Subunit - genetics; dexamethasone; Dexamethasone - pharmacology; Glycerophosphates - pharmacology; Human periodontal ligament cells; Humans; Osteocalcin - genetics; Osteogenesis - drug effects; Osteopontin - genetics; Periodontal Ligament - cytology; Periodontal Ligament - drug effects; Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - genetics; β-glycerophosphate
• ISSN: 0897-7194; 1029-2292
• DOI: 10.3109/08977194.2010.495719

This study was designed to investigate the potential merits of the combined use of bone morphogenetic protein (BMP)-2 or BMP-6 and osteogenic supplements (OS) [dexamethasone, ascorbic acid (AA), and β-glycerophosphate] on osteogenic differentiation of periodontal ligament cells (PDLCs). Osteogenic differentiation was evaluated by quantitative alkaline phosphatase (ALP) assay, alizarin red staining, quantitative calcium assay, and the qRT-PCR analysis for the expression of collagen type I, runt-related transcription factor-2, osteopontin (OPN), and osteocalcin in PDLCs. Culture with BMP-2 or BMP-6+AA increased ALP activity of PDLCs, suggesting their osteo-inductive effects. However, longer duration of culture showed neither of the BMPs induced in vitro mineralization. In contrast, OS were able to increase ALP activity and OPN expressions, and also induced in vitro mineralization. The mineralization ability was not enhanced by the addition of BMP-2 or BMP-6. These findings suggest that the addition of BMP-2 or BMP-6 to OS may not enhance an osteogenic differentiation of hPDLCs.