A Highly Sensitive Molecular Technique for RNA Virus Detection
Zika (ZIKV) and Chikungunya (CHIKV) viruses are mosquito-transmitted infections, or vector-borne pathogens, that emerged a few years ago. Reliable diagnostic tools for ZIKV and CHIKV-inexpensive, multiplexed, rapid, highly sensitive, and specific point-of-care (POC) systems-are vital for appropriate...
Saved in:
| Published in: | Cells (Basel, Switzerland) Vol. 13; no. 10; p. 804 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
MDPI AG
09-05-2024
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Zika (ZIKV) and Chikungunya (CHIKV) viruses are mosquito-transmitted infections, or vector-borne pathogens, that emerged a few years ago. Reliable diagnostic tools for ZIKV and CHIKV-inexpensive, multiplexed, rapid, highly sensitive, and specific point-of-care (POC) systems-are vital for appropriate risk management and therapy. We recently studied a detection system with great success in Mexico (Villahermosa, state of Tabasco), working with human sera from patients infected with those viruses. The research conducted in Mexico validated the efficacy of a novel two-step rapid isothermal amplification technique (RAMP). This approach, which encompasses recombinase polymerase amplification (RPA) followed by loop-mediated isothermal amplification (LAMP), had been previously established in the lab using lab-derived Zika (ZIKV) and Chikungunya (CHIKV) viruses. Crucially, our findings confirmed that this technique is also effective when applied to human sera samples collected from locally infected individuals in Mexico. |
|---|---|
| AbstractList | Zika (ZIKV) and Chikungunya (CHIKV) viruses are mosquito-transmitted infections, or vector-borne pathogens, that emerged a few years ago. Reliable diagnostic tools for ZIKV and CHIKV-inexpensive, multiplexed, rapid, highly sensitive, and specific point-of-care (POC) systems-are vital for appropriate risk management and therapy. We recently studied a detection system with great success in Mexico (Villahermosa, state of Tabasco), working with human sera from patients infected with those viruses. The research conducted in Mexico validated the efficacy of a novel two-step rapid isothermal amplification technique (RAMP). This approach, which encompasses recombinase polymerase amplification (RPA) followed by loop-mediated isothermal amplification (LAMP), had been previously established in the lab using lab-derived Zika (ZIKV) and Chikungunya (CHIKV) viruses. Crucially, our findings confirmed that this technique is also effective when applied to human sera samples collected from locally infected individuals in Mexico. |
| Audience | Academic |
| Author | León-Juárez, Moisés Arévalo-Romero, Haruki Rozmyslowicz, Tomasz Fuentes-Pananá, Ezequiel M Conover, Dareus O Gaulton, Glen N |
| Author_xml | – sequence: 1 givenname: Tomasz surname: Rozmyslowicz fullname: Rozmyslowicz, Tomasz organization: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 2 givenname: Haruki orcidid: 0000-0002-4768-1814 surname: Arévalo-Romero fullname: Arévalo-Romero, Haruki organization: Laboratorio de Inmunología y Microbiología Molecular, División Académica Multidisciplinaria de Jalpa de Méndez, Departamento de Genómica, Universidad Juárez Autónoma de Tabasco, Jalpa de Méndez 86205, Mexico – sequence: 3 givenname: Dareus O surname: Conover fullname: Conover, Dareus O organization: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA – sequence: 4 givenname: Ezequiel M surname: Fuentes-Pananá fullname: Fuentes-Pananá, Ezequiel M organization: Unidad de Investigación en Virología y Cáncer, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, Mexico – sequence: 5 givenname: Moisés orcidid: 0000-0002-5726-5953 surname: León-Juárez fullname: León-Juárez, Moisés organization: Laboratorio de Virología Perinatal, Departamento de Inmunobioquímica, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Ciudad de México 06720, Mexico – sequence: 6 givenname: Glen N surname: Gaulton fullname: Gaulton, Glen N organization: Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38786028$$D View this record in MEDLINE/PubMed |
| BookMark | eNptkc1PFTEUxRsDEUSWbs0kbtwMtL2dfmxMXhCFBDRRdNt0-vHoy7wptjMm_Pf0-fBFDO2izc3vnt7T8wrtjWn0CL0h-ARA4VPrh6EQIBhLzF6gQ4oFtIxhtffP_QAdl7LCdUnCCe5eogOQQnJM5SH6sGgu4vJ2uG---7HEKf72zXUavJ0Hk5sbb2_H-Gv2TUi5-fZl0fyMeS7NRz95O8U0vkb7wQzFHz-eR-jHp_Obs4v26uvny7PFVWsZU1MbXM-IDdB3znlJwNW5CARKjeJMCEyNk4oCp9iGwFxPOSGiF51VigdhezhCl1tdl8xK3-W4NvleJxP1n0LKS23yFO3gteEds6F3rgfMOmWMcIxTsJ7yIAMLVev9Vusup2qtTHody-YnzejTXDRgjkFI1omKvvsPXaU5j9VppToFjHbVzY5amvp-HEOasrEbUb0QqgMlCYFKnTxD1e38Otqaa4i1_qSh3TbYnErJPux8E6w38esn8Vf-7eOwc7_2bkf_DRseAIZVqOY |
| Cites_doi | 10.3389/fmicb.2021.615165 10.1371/journal.pbio.0040204 10.1186/s12985-016-0556-9 10.1016/j.jviromet.2016.10.010 10.3201/eid2101.140894 10.1080/21505594.2021.1996059 10.1126/science.318.5858.1860 10.3201/eid2111.150546 10.1038/s41598-023-35143-2 10.1093/nar/28.12.e63 10.1038/nrmicro.2017.170 10.1039/c1an00007a 10.1016/j.virol.2009.07.024 10.3201/eid1909.130130 10.3390/v11111078 10.1038/s41598-020-75891-z 10.1371/annotation/850ee20f-2641-46ac-b0c6-ef4ae79b6de6 10.1186/s12879-017-2382-0 10.2807/1560-7917.ES.2016.21.26.30269 10.1016/j.cell.2017.03.016 10.1056/NEJMp1407698 10.1172/JCI84417 10.1016/S0140-6736(16)00562-6 10.1371/journal.ppat.0030089 10.1038/s41598-020-68469-2 10.1073/pnas.1713166114 10.1039/c1lc20345b 10.3389/fimmu.2019.01617 10.1186/1743-422X-7-327 10.1371/journal.pone.0212114 10.1371/journal.pntd.0004318 10.1016/j.chom.2018.04.017 10.1039/C5LC01235J 10.1101/2023.03.08.22283959 10.1126/science.aaf5316 10.1056/NEJMsr1604338 10.1038/srep38223 10.1016/j.cell.2014.10.004 10.1021/acs.analchem.6b01632 10.1056/NEJMoa1601824 10.1016/j.coviro.2011.07.004 10.1038/s41598-017-13836-9 10.1016/j.snb.2016.01.073 10.1016/S0140-6736(11)60281-X 10.1016/S1473-3099(16)00138-9 10.1016/S0140-6736(16)00003-9 10.4049/jimmunol.0900255 10.1016/j.cell.2016.04.059 10.1056/NEJMoa1602412 10.1056/NEJMp1408509 10.1371/journal.pone.0042222 10.1016/j.cell.2019.04.008 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2024 MDPI AG 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: COPYRIGHT 2024 MDPI AG – notice: 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 8FD 8FE 8FH ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FR3 GNUQQ HCIFZ LK8 M7P P64 PIMPY PQEST PQQKQ PQUKI PRINS RC3 7X8 DOA |
| DOI | 10.3390/cells13100804 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Engineering Research Database ProQuest Central Student SciTech Premium Collection Biological Sciences Biological Science Database Biotechnology and BioEngineering Abstracts Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts MEDLINE - Academic DOAJ Directory of Open Access Journals |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Publicly Available Content Database ProQuest Central Student Technology Research Database ProQuest Biological Science Collection ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central China Biotechnology and BioEngineering Abstracts ProQuest Central Genetics Abstracts ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection Engineering Research Database ProQuest One Academic MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE CrossRef Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2073-4409 |
| ExternalDocumentID | oai_doaj_org_article_a654cfbddb30459aa7d4623ce26f8f4f A795398113 10_3390_cells13100804 38786028 |
| Genre | Journal Article |
| GeographicLocations | Mexico Africa |
| GeographicLocations_xml | – name: Mexico – name: Africa |
| GrantInformation_xml | – fundername: NIH HHS grantid: NIH 1 R21 AI134594-01A1. |
| GroupedDBID | 53G 5VS 8FE 8FH AADQD AAFWJ ABDBF ADBBV AFKRA AFPKN AFZYC ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BBNVY BCNDV BENPR BHPHI CCPQU CGR CUY CVF DIK EBD ECM EIF ESX GROUPED_DOAJ HCIFZ HYE IAO IHR ITC KQ8 LK8 M48 M7P MODMG M~E NPM OK1 PGMZT PIMPY PROAC RIG RPM AAYXX CITATION 8FD ABUWG AZQEC DWQXO FR3 GNUQQ P64 PQEST PQQKQ PQUKI PRINS RC3 7X8 |
| ID | FETCH-LOGICAL-c449t-fdb41cf3b5dde813d44013f22a9647702ad8923620cff4db26117b75c996f7cb3 |
| IEDL.DBID | DOA |
| ISSN | 2073-4409 |
| IngestDate | Tue Oct 22 15:16:08 EDT 2024 Sat Oct 26 05:11:30 EDT 2024 Thu Oct 10 17:19:33 EDT 2024 Tue Nov 19 21:35:00 EST 2024 Tue Nov 12 23:39:40 EST 2024 Thu Nov 21 22:37:13 EST 2024 Sat Nov 02 12:25:46 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Keywords | Chikungunya detection diagnostics LAMP Zika |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c449t-fdb41cf3b5dde813d44013f22a9647702ad8923620cff4db26117b75c996f7cb3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0002-5726-5953 0000-0002-4768-1814 |
| OpenAccessLink | https://doaj.org/article/a654cfbddb30459aa7d4623ce26f8f4f |
| PMID | 38786028 |
| PQID | 3059342581 |
| PQPubID | 2032536 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_a654cfbddb30459aa7d4623ce26f8f4f proquest_miscellaneous_3060378457 proquest_journals_3059342581 gale_infotracmisc_A795398113 gale_infotracacademiconefile_A795398113 crossref_primary_10_3390_cells13100804 pubmed_primary_38786028 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-05-09 |
| PublicationDateYYYYMMDD | 2024-05-09 |
| PublicationDate_xml | – month: 05 year: 2024 text: 2024-05-09 day: 09 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | Cells (Basel, Switzerland) |
| PublicationTitleAlternate | Cells |
| PublicationYear | 2024 |
| Publisher | MDPI AG |
| Publisher_xml | – name: MDPI AG |
| References | Staples (ref_11) 2014; 371 ref_50 ref_54 Gourinat (ref_40) 2015; 21 Wang (ref_55) 2016; 238 Thomas (ref_22) 2010; 7 (ref_15) 2019; 10 Sirohi (ref_6) 2016; 352 Richner (ref_14) 2017; 169 Pardee (ref_44) 2014; 159 ref_21 ref_20 Song (ref_52) 2016; 88 Hoarau (ref_29) 2010; 184 Horwood (ref_10) 2013; 19 ref_28 Liu (ref_32) 2011; 136 Morens (ref_16) 2014; 371 ref_26 Chan (ref_53) 2016; 6 Burt (ref_27) 2012; 379 Kautz (ref_51) 2015; 21 ref_36 Kurosaki (ref_2) 2017; 7 Song (ref_19) 2019; 177 ref_31 ref_30 Li (ref_48) 2018; 23 Solignat (ref_18) 2009; 393 Neufeldt (ref_8) 2018; 16 Sharma (ref_1) 2020; 10 ref_37 Lustig (ref_41) 2016; 21 Sarti (ref_4) 2020; 10 Musso (ref_42) 2016; 13 Blake (ref_13) 2016; 387 Rasmussen (ref_12) 2016; 374 Silva (ref_17) 2017; 127 Tsetsarkin (ref_25) 2011; 1 Notomi (ref_46) 2000; 28 Enfissi (ref_38) 2016; 387 Driggers (ref_43) 2016; 374 ref_47 Yap (ref_23) 2017; 114 Liu (ref_35) 2016; 16 Mansuy (ref_39) 2016; 16 Liao (ref_34) 2016; 229 Liu (ref_33) 2011; 11 Shimelis (ref_3) 2023; 13 Heunis (ref_7) 2021; 12 Brasil (ref_9) 2016; 375 Enserink (ref_24) 2007; 318 ref_49 ref_5 Pardee (ref_45) 2016; 165 |
| References_xml | – ident: ref_20 doi: 10.3389/fmicb.2021.615165 – ident: ref_5 – ident: ref_47 doi: 10.1371/journal.pbio.0040204 – volume: 13 start-page: 102 year: 2016 ident: ref_42 article-title: Detection of chikungunya virus in saliva and urine publication-title: Virol. J. doi: 10.1186/s12985-016-0556-9 contributor: fullname: Musso – volume: 238 start-page: 86 year: 2016 ident: ref_55 article-title: Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification publication-title: J. Virol. Methods doi: 10.1016/j.jviromet.2016.10.010 contributor: fullname: Wang – volume: 21 start-page: 84 year: 2015 ident: ref_40 article-title: Detection of Zika virus in urine publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2101.140894 contributor: fullname: Gourinat – volume: 12 start-page: 2814 year: 2021 ident: ref_7 article-title: Pathogenesis and virulence of flavivirus infections publication-title: Virulence doi: 10.1080/21505594.2021.1996059 contributor: fullname: Heunis – volume: 318 start-page: 1860 year: 2007 ident: ref_24 article-title: Infectious diseases. Chikungunya: No longer a third world disease publication-title: Science doi: 10.1126/science.318.5858.1860 contributor: fullname: Enserink – volume: 21 start-page: 2070 year: 2015 ident: ref_51 article-title: Chikungunya Virus as Cause of Febrile Illness Outbreak, Chiapas, Mexico, 2014 publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2111.150546 contributor: fullname: Kautz – volume: 13 start-page: 7997 year: 2023 ident: ref_3 article-title: Detection of dengue virus infection in children presenting with fever in Hawassa, southern Ethiopia publication-title: Sci. Rep. doi: 10.1038/s41598-023-35143-2 contributor: fullname: Shimelis – volume: 28 start-page: E63 year: 2000 ident: ref_46 article-title: Loop-mediated isothermal amplification of DNA publication-title: Nucleic Acids Res. doi: 10.1093/nar/28.12.e63 contributor: fullname: Notomi – ident: ref_31 – volume: 16 start-page: 125 year: 2018 ident: ref_8 article-title: Rewiring cellular networks by members of the Flaviviridae family publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro.2017.170 contributor: fullname: Neufeldt – volume: 136 start-page: 2069 year: 2011 ident: ref_32 article-title: An isothermal amplification reactor with an integrated isolation membrane for point-of-care detection of infectious diseases publication-title: Analyst doi: 10.1039/c1an00007a contributor: fullname: Liu – volume: 393 start-page: 183 year: 2009 ident: ref_18 article-title: Replication cycle of chikungunya: A re-emerging arbovirus publication-title: Virology doi: 10.1016/j.virol.2009.07.024 contributor: fullname: Solignat – volume: 19 start-page: 1535 year: 2013 ident: ref_10 article-title: Outbreak of chikungunya virus infection, Vanimo, Papua New Guinea publication-title: Emerg. Infect. Dis. doi: 10.3201/eid1909.130130 contributor: fullname: Horwood – ident: ref_21 doi: 10.3390/v11111078 – volume: 10 start-page: 19017 year: 2020 ident: ref_4 article-title: Risk of dengue virus infection according to serostatus in individuals from dengue endemic areas of Mexico publication-title: Sci. Rep. doi: 10.1038/s41598-020-75891-z contributor: fullname: Sarti – ident: ref_26 doi: 10.1371/annotation/850ee20f-2641-46ac-b0c6-ef4ae79b6de6 – ident: ref_54 doi: 10.1186/s12879-017-2382-0 – volume: 21 start-page: 30269 year: 2016 ident: ref_41 article-title: Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016 publication-title: Euro Surveill. doi: 10.2807/1560-7917.ES.2016.21.26.30269 contributor: fullname: Lustig – volume: 169 start-page: 176 year: 2017 ident: ref_14 article-title: Modified mRNA Vaccines Protect against Zika Virus Infection publication-title: Cell doi: 10.1016/j.cell.2017.03.016 contributor: fullname: Richner – volume: 371 start-page: 887 year: 2014 ident: ref_11 article-title: Chikungunya virus in the Americas—What a vectorborne pathogen can do publication-title: N. Engl. J. Med. doi: 10.1056/NEJMp1407698 contributor: fullname: Staples – volume: 127 start-page: 737 year: 2017 ident: ref_17 article-title: Chikungunya virus: Epidemiology, replication, disease mechanisms, and prospective intervention strategies publication-title: J. Clin. Investig. doi: 10.1172/JCI84417 contributor: fullname: Silva – volume: 387 start-page: 1531 year: 2016 ident: ref_13 article-title: Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: A case-control study publication-title: Lancet doi: 10.1016/S0140-6736(16)00562-6 contributor: fullname: Blake – ident: ref_28 doi: 10.1371/journal.ppat.0030089 – volume: 10 start-page: 11651 year: 2020 ident: ref_1 article-title: Development of magnetic bead based sample extraction coupled polymerase spiral reaction for rapid on-site detection of Chikungunya virus publication-title: Sci. Rep. doi: 10.1038/s41598-020-68469-2 contributor: fullname: Sharma – volume: 114 start-page: 13703 year: 2017 ident: ref_23 article-title: Structural studies of Chikungunya virus maturation publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1713166114 contributor: fullname: Yap – ident: ref_30 – volume: 11 start-page: 2686 year: 2011 ident: ref_33 article-title: A self-heating cartridge for molecular diagnostics publication-title: Lab Chip doi: 10.1039/c1lc20345b contributor: fullname: Liu – volume: 10 start-page: 1617 year: 2019 ident: ref_15 article-title: The Dual Role of the Immune Response in Reproductive Organs During Zika Virus Infection publication-title: Front. Immunol. doi: 10.3389/fimmu.2019.01617 – volume: 7 start-page: 327 year: 2010 ident: ref_22 article-title: Functional dissection of the alphavirus capsid protease: Sequence requirements for activity publication-title: Virol. J. doi: 10.1186/1743-422X-7-327 contributor: fullname: Thomas – ident: ref_50 doi: 10.1371/journal.pone.0212114 – ident: ref_36 doi: 10.1371/journal.pntd.0004318 – volume: 23 start-page: 855 year: 2018 ident: ref_48 article-title: Genomic Epidemiology Reconstructs the Introduction and Spread of Zika Virus in Central America and Mexico publication-title: Cell Host Microbe doi: 10.1016/j.chom.2018.04.017 contributor: fullname: Li – volume: 16 start-page: 553 year: 2016 ident: ref_35 article-title: A high-efficiency superhydrophobic plasma separator publication-title: Lab Chip doi: 10.1039/C5LC01235J contributor: fullname: Liu – ident: ref_49 doi: 10.1101/2023.03.08.22283959 – volume: 352 start-page: 467 year: 2016 ident: ref_6 article-title: The 3.8 Å resolution cryo-EM structure of Zika virus publication-title: Science doi: 10.1126/science.aaf5316 contributor: fullname: Sirohi – volume: 374 start-page: 1981 year: 2016 ident: ref_12 article-title: Zika Virus and Birth Defects—Reviewing the Evidence for Causality publication-title: N. Engl. J. Med. doi: 10.1056/NEJMsr1604338 contributor: fullname: Rasmussen – volume: 6 start-page: 38223 year: 2016 ident: ref_53 article-title: Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus publication-title: Sci. Rep. doi: 10.1038/srep38223 contributor: fullname: Chan – volume: 159 start-page: 940 year: 2014 ident: ref_44 article-title: Paper-based synthetic gene networks publication-title: Cell doi: 10.1016/j.cell.2014.10.004 contributor: fullname: Pardee – volume: 88 start-page: 7289 year: 2016 ident: ref_52 article-title: Instrument-Free Point-of-Care Molecular Detection of Zika Virus publication-title: Anal. Chem. doi: 10.1021/acs.analchem.6b01632 contributor: fullname: Song – volume: 374 start-page: 2142 year: 2016 ident: ref_43 article-title: Zika Virus Infection with Prolonged Maternal Viremia and Fetal Brain Abnormalities publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1601824 contributor: fullname: Driggers – volume: 1 start-page: 310 year: 2011 ident: ref_25 article-title: Chikungunya virus: Evolution and genetic determinants of emergence publication-title: Curr. Opin. Virol. doi: 10.1016/j.coviro.2011.07.004 contributor: fullname: Tsetsarkin – volume: 7 start-page: 13503 year: 2017 ident: ref_2 article-title: Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification publication-title: Sci. Rep. doi: 10.1038/s41598-017-13836-9 contributor: fullname: Kurosaki – volume: 229 start-page: 232 year: 2016 ident: ref_34 article-title: Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostic Device publication-title: Sens. Actuators B Chem. doi: 10.1016/j.snb.2016.01.073 contributor: fullname: Liao – volume: 379 start-page: 662 year: 2012 ident: ref_27 article-title: Chikungunya: A re-emerging virus publication-title: Lancet doi: 10.1016/S0140-6736(11)60281-X contributor: fullname: Burt – volume: 16 start-page: 405 year: 2016 ident: ref_39 article-title: Zika virus: High infectious viral load in semen, a new sexually transmitted pathogen? publication-title: Lancet Infect. Dis. doi: 10.1016/S1473-3099(16)00138-9 contributor: fullname: Mansuy – volume: 387 start-page: 227 year: 2016 ident: ref_38 article-title: Zika virus genome from the Americas publication-title: Lancet doi: 10.1016/S0140-6736(16)00003-9 contributor: fullname: Enfissi – volume: 184 start-page: 5914 year: 2010 ident: ref_29 article-title: Persistent chronic inflammation and infection by Chikungunya arthritogenic alphavirus in spite of a robust host immune response publication-title: J. Immunol. doi: 10.4049/jimmunol.0900255 contributor: fullname: Hoarau – volume: 165 start-page: 1255 year: 2016 ident: ref_45 article-title: Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components publication-title: Cell doi: 10.1016/j.cell.2016.04.059 contributor: fullname: Pardee – volume: 375 start-page: 2321 year: 2016 ident: ref_9 article-title: Zika Virus Infection in Pregnant Women in Rio de Janeiro publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1602412 contributor: fullname: Brasil – volume: 371 start-page: 885 year: 2014 ident: ref_16 article-title: Chikungunya at the door—Déjà vu all over again? publication-title: N. Engl. J. Med. doi: 10.1056/NEJMp1408509 contributor: fullname: Morens – ident: ref_37 doi: 10.1371/journal.pone.0042222 – volume: 177 start-page: 1714 year: 2019 ident: ref_19 article-title: Molecular Basis of Arthritogenic Alphavirus Receptor MXRA8 Binding to Chikungunya Virus Envelope Protein publication-title: Cell doi: 10.1016/j.cell.2019.04.008 contributor: fullname: Song |
| SSID | ssj0000816105 |
| Score | 2.3188627 |
| Snippet | Zika (ZIKV) and Chikungunya (CHIKV) viruses are mosquito-transmitted infections, or vector-borne pathogens, that emerged a few years ago. Reliable diagnostic... |
| SourceID | doaj proquest gale crossref pubmed |
| SourceType | Open Website Aggregation Database Index Database |
| StartPage | 804 |
| SubjectTerms | Blood & organ donations Chikungunya Chikungunya Fever - blood Chikungunya Fever - diagnosis Chikungunya Fever - virology Chikungunya virus Chikungunya virus - genetics Chikungunya virus - isolation & purification Dengue fever detection Detectors diagnostics Disease management Disease transmission Endoplasmic reticulum Epidemics Ethylenediaminetetraacetic acid Gene amplification Genomes Guillain-Barre syndrome Health aspects Humans Illnesses Infection Infections Kinases LAMP Methods Mexico Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Pathogens Proteins Recombinase RNA RNA viruses RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Viral - blood RNA, Viral - genetics Sensitivity and Specificity Vaccines Womens health Zika Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - blood Zika Virus Infection - diagnosis Zika Virus Infection - virology |
| Title | A Highly Sensitive Molecular Technique for RNA Virus Detection |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38786028 https://www.proquest.com/docview/3059342581 https://www.proquest.com/docview/3060378457 https://doaj.org/article/a654cfbddb30459aa7d4623ce26f8f4f |
| Volume | 13 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3Na90wDBddYdDL2Ee3Ze2KB6M9hSaxHTuXwdva0lHWQ9uN3Yw_oTDS8T4O_e8rOcljrzvssmusgC3ZspRIvx_Ax7qyMgpqjkF7l4LrUOrkZal8pxqpY9Vaak4-v1aXP_XJKcHkrKm-qCZsgAceFHdsWyl8ciE4-qfXWauCwCvbx6ZNOomUvW_V_pFMZR-sMZKp5ACqyTGvP6bv4IuaPmfrkZRtuoQyVv_fHvlRnJnvm7Pn8GwMFNlsmOAL2Ir9S3g6UEfev4JPM0YlGr_u2TWVoJPTYt8mqlt2MyGzMoxJ2dXljP24na8W7CQuc-lVvwvfz05vvpyXIxdC6YXolmUKTtQ-cSfRH-maB0GJUWoam1tJq8YGjbFa21Q-JREcJka1ckp6zGeS8o6_hu3-ro9vgXGJKYcnURWFltYKlyK3LQZyQTirCjiclGN-D5AXBlMF0qLZ0GIBn0l1ayFCqs4P0H5mtJ_5l_0KOCLFGzpPy7n1dmwLwLkSMpWZqU7yTtc1L2B_QxLPgd8cnkxnxnO4MJwYC9Et6bqAD-thepNqy_p4tyKZtuJKC4krfzOYfL0krhWRdOl3_2Ope7DTYEiUyyW7fdhezlfxPTxZhNUB7t6vFwd5Dz8A_1ryUg |
| link.rule.ids | 315,782,786,866,2106,27933,27934 |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Highly+Sensitive+Molecular+Technique+for+RNA+Virus+Detection&rft.jtitle=Cells+%28Basel%2C+Switzerland%29&rft.au=Rozmyslowicz%2C+Tomasz&rft.au=Ar%C3%A9valo-Romero%2C+Haruki&rft.au=Conover%2C+Dareus+O&rft.au=Fuentes-Panan%C3%A1%2C+Ezequiel+M&rft.date=2024-05-09&rft.eissn=2073-4409&rft.volume=13&rft.issue=10&rft_id=info:doi/10.3390%2Fcells13100804&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2073-4409&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2073-4409&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2073-4409&client=summon |