Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage

• Published in: Chemical science (Cambridge) Vol. 6; no. 11; pp. 6270 - 6279
• Main Authors: Tang, Weixin, Dong, Shi-Hui, Repka, Lindsay M, He, Chang, Nair, Satish K, van der Donk, Wilfred A
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 01-01-2015
• Subjects: Amino acids; Biosynthesis; Biotechnology; Bonding; Cleavage; Crystal structure; Peptides; Protease
• ISSN: 2041-6520; 2041-6539
• DOI: 10.1039/c5sc02329g

The final step of lanthipeptide biosynthesis involves the removal of leader peptides by dedicated proteases.
characterization of LicP, a class II LanP protease involved in the biosynthesis of the lantibiotic lichenicidin, revealed a self-cleavage step that removes 100 amino acids from the N-terminus. The 2.35 Å resolution crystal structure provides insights into the active site geometry and substrate specificity, and unveiled an unusual calcium-independent maturation mechanism of a subtilisin family member. LicP processes LicA2 peptides with or without post-translational modifications, but dehydrated and cyclized LicA2 is favored. Investigation of its substrate specificity demonstrated that LicP can serve as an efficient sequence-specific traceless protease and may have great utility in basic research and biotechnology. Encouraged by these findings for LicP, we identified 13 other class II LanPs, ten of which were previously unknown, and suggest that these proteins may serve as a pool of proteases with diverse recognition sequences for general traceless tag removal applications, expanding the current toolbox of proteases.