Complete mitochondrial DNA of Hoplias intermedius (Günther, 1864) (Ostariophysi: Characiformes: Erythrinidae)

• Published in: Mitochondrial DNA. Part B. Resources Vol. 1; no. 1; pp. 742 - 743
• Main Authors: Núñez Rodriguez, Daniela, Lucio, Nazaré, Costa, Maísa Aparecida, Resende, Leonardo, Martins, Ana Paula Vimieiro, Alves, Carlos Bernardo Mascarenhas, Carmo, Anderson Oliveira do, Kalapothakis, Evanguedes
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-01-2016; Taylor & Francis Ltd
• Subjects: "traírão"; Adenosine triphosphatase; Characiformes; Codons; Deoxyribonucleic acid; DNA; DNA sequencing; Genomes; Hoplias intermedius; Mitochondrial DNA; Mitogenome Announcement; mtDNA; Next-generation sequencing; Nucleotide sequence; Ostariophysi; Phylogenetics; Phylogeny; rRNA; São Francisco river basin; tRNA; Next-generation sequencing; traírão; mtDNA; São Francisco river basin
• ISSN: 2380-2359; 2380-2359
• DOI: 10.1080/23802359.2016.1186521

Mitochondrial complete genome (mtDNA) of Hoplias intermedius, commonly known as "traírão" is presented. DNA from a muscle tissue sample was sequenced by next-generation sequencing. To corroborate the results, phylogenetic analysis was performed with MEGA6 to compare the complete mitogenome of H. intermedius with genomes of other Characiformes species. The mtDNA molecule was circular and 16,629 bp in length. Gene content included 22 tRNA genes, 2 rRNA genes, 13 protein-coding genes and a D-loop noncoding region. All genes had ATG as the start codon. Six genes carried complete stop codons of AGG (COI), TGA (ND6) and TAA (ND1, ATPase 8, ND4L and ND5), while seven genes had incomplete stop codons. Phylogenetic relationships with other Ostariophysi species placed H. intermedius together with H. malabaricus as a monophyletic group belonging to the order Characiformes.