The Cryoprotective Effect of an Antifreeze Collagen Peptide Complex Obtained by Enzymatic Glycosylation on Tilapia

• Published in: Foods Vol. 13; no. 9; p. 1319
• Main Authors: Liu, Shouchun, Zhang, Luyao, Li, Zhuyi, Chen, Jing, Zhang, Yinyu, Yang, Xuebo, Chen, Qiuhan, Cai, Hongying, Hong, Pengzhi, Zhu, Chunhua, Zhong, Saiyi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-05-2024
• Subjects: Amino acids; antifreeze; Antifreezes; Backup software; Biotechnology; Collagen; Denaturation; Fish; fish collagen peptide; Fluorescence in situ hybridization; Fluorescence spectroscopy; Food; Food quality; Frozen food; Frozen foods; Frozen products; Glucosamine; Glycosylation; Meat; Methods; Muscles; Nitrogen; NMR; Nuclear magnetic resonance; Peptides; Polymers; Protein interaction; Protein structure; Proteins; Refrigeration equipment; Rheological properties; Temperature; Tertiary structure; Tilapia; transglutaminase; China; tilapia; transglutaminase; glycosylation; fish collagen peptide; antifreeze
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13091319

Antifreeze peptides have become effective antifreeze agents for frozen products, but their low quantity of active ingredients and high cost limit large-scale application. This study used the glycosylation of fish collagen peptides with glucosamine hydrochloride catalyzed by transglutaminase to obtain a transglutaminase-catalyzed glycosylation product (TGP) and investigate its antifreeze effect on tilapia. Compared with the blank group, the freshness (pH value of 6.31, TVB-N value of 21.7 mg/100 g, whiteness of 46.28), textural properties (especially hardness and elasticity), and rheological properties of the TGP groups were significantly improved. In addition, the protein structures of the samples were investigated using UV absorption and fluorescence spectroscopy. The results showed that the tertiary structure of the TGP groups changed to form a dense polymer. Therefore, this approach can reduce the denaturation and decomposition of muscle fibers and proteins in fish meat more effectively and has a better protective effect on muscle structure and protein aggregation, improving the stability of fish meat. This study reveals an innovative method for generating antifreeze peptides by enzymatic glycosylation, and glycosylated fish collagen peptide products can be used as new and effective green antifreeze agents in frozen foods.