Study of urinary 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in diabetic nephropathy patients

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 36; no. 1; pp. 101 - 104
• Main Authors: Xu, G.W., Yao, Q.H., Weng, Q.F., Su, B.L., Zhang, X., Xiong, J.H.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21-09-2004; Elsevier Science
• Subjects: 8-Hydroxydeoxyguanosine; Adult; Aged; Aged, 80 and over; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Biomarkers - urine; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - urine; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - urine; Diabetic Nephropathies - etiology; Diabetic Nephropathies - urine; Diabetic nephropathy; DNA Damage; Electrophoresis, Capillary; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Male; Medical sciences; Middle Aged; Oxidative DNA damage; Pharmacology. Drug treatments; 8-Hydroxydeoxyguanosine; Diabetic nephropathy; Oxidative DNA damage; Endocrinopathy; Creatinine; Human; Urine; Solid phase extraction; Biological fluid; Oxidative stress; Healthy subject; Capillary electrophoresis; Pathogenesis; Diabetes mellitus; Biological marker; Lipids; Triglyceride; Hyperglycemia; Hemoglobin; Complication; Hemodynamics; Lesion
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2004.04.016

Increased oxidative stress induced by hyperglycemia may contribute to the pathogenesis of diabetic complications. Urinary 8-hydroxydeoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. This article studied oxidative DNA damage in patients with diabetic nephropathy and in healthy control subjects by urinary 8-OHdG evaluations. Contents of 8-OHdG in urine were analyzed by capillary electrophoresis with end-column amperometric detection (CE-AD) after a single-step solid-phase extraction (SPE). Levels of urinary 8-OHdG in diabetic nephropathy patients with macroalbuminuria was significant higher than in control subjects (5.72±6.89
μmol/mol creatinine versus 2.33±2.83
μmol/mol creatinine,
P = 0.018). A significant difference of 24
h urinary 8-OHdG excretions exists between the patients with macroalbuminuria and the patients with normoalbuminuria (19.2±16.8
μg/24
h versus 8.1±1.7
μg/24
h,
P = 0.015). There was a positive correlation between urinary excretion of 8-OHdG and glycosylated hemoglobin (HbA
1c) (
r = 0.287,
P = 0.022). A weak correlation exists between the levels of 8-OHdG and triglyceride (
r = 0.230,
P = 0.074). However, the urinary 8-OHdG contents are not correlated with blood pressure and total cholesterol. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in diabetic nephropathy patients.