OPTIMIZATION, KINETICS, AND MODELING OF INULINASE PRODUCTION BY K. marxianus var. marxianus

• Published in: Preparative biochemistry & biotechnology Vol. 44; no. 3; pp. 291 - 309
• Main Authors: Dilipkumar, M., Rajasimman, M., Rajamohan, N.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 03-04-2014; Taylor & Francis Ltd
• Subjects: Beef; Computer Simulation; Culture Media - metabolism; Enzyme kinetics; Fermentation; Glycoside Hydrolases - metabolism; Industrial Microbiology - methods; inulinase; K. marxianus var. marxianus; Kinetics; Kluyveromyces - enzymology; Kluyveromyces - metabolism; Kluyveromyces marxianus; Models, Biological; Neural networks; Neural Networks (Computer); Optimization; pressmud; RSM; Sugar industry; Sugarcane
• ISSN: 1532-2297; 1082-6068; 1532-2297
• DOI: 10.1080/10826068.2013.812567

Pressmud, a by-product from the sugarcane industry, was used as a carbon source for the production of inulinase in solid-state fermentation (SSF). Statistical experimental designs were employed to screen the nutrients and optimize the media composition for the production of inulinase by Kluyveromyces marxianus var. marxianus . Eighteen various nutrients were selected for preliminary screening of production medium component by Plackett–Burman design (PBD) technique. Five nutrients were found to be significant for inulinase production and they were optimized by central composite design (CCD). The optimal media components for solid-state fermentation of inulinase using pressmud were (g/gds): corn steep liquor, 0.06072; urea, 0.01916; beef extract, 0.00957; FeSO ₄ · 7H ₂O, 0.00013; K ₂HPO ₄, 0.00441. The effect of moisture content and substrate concentration was also studied. From the results it was found that a maximum inulinase activity of 288 U/gds occurs at the moisture content of 65% and substrate concentration of 10 g. The constants in the Michaelis–Menten equation were evaluated and a high R ² value implied the fitness of the model. Artificial neural network (ANN) modeling was also employed to predict the inulinase production.