Chronic weight cycling increases oxidative DNA damage levels in mammary gland of female rats fed a high-fat diet

• Published in: Nutrition and cancer Vol. 29; no. 1; pp. 55 - 59
• Main Authors: Uhley, V.E, Pellizzon, M.A, Buison, A.M, Guo, F, Djuric, Z, Jen, K.J.C
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 1997; Taylor& Francis
• Subjects: 5-HYDROXYMETHYL-2-DEOXYURIDINE; ADN; ALIMENTACION RACIONADA; ALIMENTATION RATIONNEE; Animals; Biological and medical sciences; BODY WEIGHT; CALORIC INTAKE; Carcinogenesis, carcinogens and anticarcinogens; CORPS GRAS; CYCLIC FLUCTUATIONS; DAMAGE; DANOS; DEGAT; DEOXYRIBONUCLEOSIDE; DEOXYRIBONUCLEOSIDES; DEOXYURIDINES; DESOXIRIBONUCLEOSIDOS; DIET; DIETA; DNA; DNA Damage; ELDERLY; Energy Intake; ENERGY VALUE; FATS; Female; FEMALES; FEMELLE; Foods and miscellaneous; GLANDE MAMMAIRE; GLANDULAS MAMARIAS; GRASAS; Gynecology. Andrology. Obstetrics; HEMBRA; INGESTION DE NUTRIENTES; INGESTION DE SUBSTANCES NUTRITIVES; Mammary gland diseases; MAMMARY GLANDS; Mammary Glands, Animal - metabolism; Medical sciences; NUTRIENT INTAKE; OXIDACION; OXIDATION; Oxidation-Reduction; OXYDATION; PERIODICIDAD; PERIODICITE; PERIODICITY; PERSONNE AGEE; PESO CORPORAL; POIDS CORPOREL; RAT; RATA; RATS; Rats, Wistar; REGIME ALIMENTAIRE; RESIDU; RESIDUES; RESIDUOS; RESTRICTED FEEDING; TERCERA EDAD; THYMIDINE; Thymidine - analogs & derivatives; Thymidine - metabolism; TIMIDINA; Tumors; VALEUR ENERGETIQUE; VALOR ENERGETICO; Weight Gain; Weight Loss; Animal model; Oxidative stress; Deficient diet; Rat; Rodentia; Biological marker; Malignant tumor; Experimental study; Mammary gland diseases; Vertebrata; Mammalia; Animal; DNA; Risk factor; Fat; Mammary gland
• ISSN: 0163-5581; 1532-7914
• DOI: 10.1080/01635589709514602

Oxidative DNA damage levels may be a marker of breast cancer risk that is modulated by diet. We examined the effects of a high-fat diet fed in varying feeding regimens on levels of 5-hydroxymethyl-2'-deoxyuridine (5-OHmU), an oxidized thymidine residue, in DNA from mammary gland of aging female rats. A total of 48 rats were randomly divided into four groups: ad libitum fed (AL), weight cycled above baseline (WC-G), weight cycled below baseline (WC-L), or energy restricted (ER) for 28 weeks. WC groups were fed repeated ad libitum/restricted amounts of the diet. At sacrifice, both WC groups had body weights similar to the ER group but higher levels of 5-OHmU (p 0.01). 5-OHmU levels were higher in the WC groups than in the AL group, even though body weights of the WC groups were significantly lower (p 0.001). These results indicate that a history of weight cycling, even when body weight is reduced, can have adverse effects on 5-OHmU levels in mammary gland DNA, a potential biomarker of cancer risk. Constant control of calories for the maintenance of body weight, therefore, may be more beneficial