Real-Time Chlorophyll-a Pigment Monitoring of Chlamydomonas reinhardtii in a Controlled Environment Using Pulsed LED Fluorescence LiDAR System

• Published in: Photonics Vol. 10; no. 2; p. 144
• Main Authors: Cadondon, Jumar G., Vallar, Edgar A., Shiina, Tatsuo, Galvez, Maria Cecilia D.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01-02-2023
• Subjects: Algae; applied optics; Biomass; Chlamydomonas reinhardtii; Chlorophyll; chlorophyll-a; Cultural heritage; Emission measurements; Fluorescence; LED fluorescence LiDAR; Lidar; Light; Monitoring; Optical communication; Optical density; optical sensing; Optics; Parameters; Real time; Remote sensing; Spectrum analysis; Water quality; Wavelength; United States > US; Japan
• ISSN: 2304-6732; 2304-6732
• DOI: 10.3390/photonics10020144

The real-time chlorophyll-a pigment monitoring of C. reinhardtii is studied using our developed LED fluorescence light detection and ranging (LiDAR) system. It features a portable set-up that uses a pulsed LED module with an excitation wavelength of 385 nm. We were able to monitor the different growth phases of C. reinhardtii with specific cultivation parameters. The developed fluorescence LiDAR system showed the linear correlation of its chlorophyll-a signal with the optical density and EEM fluorescence measurements at 680 nm emission wavelength. Water quality and weather parameters were also measured, which explains the variation in the growth dynamics of C. reinhardtii during the sampling period. The results from the monitoring demonstrated a different technique that can be used in estimating algal biomass in the environment.