Derivation of DM2 affected human embryonic stem cell line Genea066

The Genea066 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying expansion of CCTG repeats in exon 1 of the ZNF9 gene, indicative of Myotonic Dystrophy Type 2 (DM2). Following ICM outgrowth on inactivated human feeders, karyotype was confi...

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Bibliographic Details
Published in:	Stem cell research Vol. 16; no. 2; pp. 497 - 499
Main Authors:	Dumevska, Biljana, Schaft, Julia, McKernan, Robert, Goel, Divya, Schmidt, Uli
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 01-03-2016 Elsevier
Subjects:	Alleles Blastocyst - cytology Cells, Cultured Comparative Genomic Hybridization Genotype Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Karyotype Male Microscopy, Fluorescence Mycoplasma Myotonic Dystrophy - metabolism Myotonic Dystrophy - pathology RNA-Binding Proteins - genetics Transcription Factors - genetics Transcription Factors - metabolism
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Summary:	The Genea066 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying expansion of CCTG repeats in exon 1 of the ZNF9 gene, indicative of Myotonic Dystrophy Type 2 (DM2). Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 88% of cells expressed Nanog, 97% Oct4, 80% Tra1-60 and 99% SSEA4 and gave a Pluritest Pluripotency score of 31.3, Novelty of 1.22. The cell line was negative for Mycoplasma and visible contamination.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2016.02.002