Structural Analysis of Fibronectin and Its Collagen-Binding Fragment from Several Cell Lines

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 1; pp. 28 - 32
• Main Author: Ali, Iqbal Unnisa
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-01-1984; National Acad Sciences
• Subjects: Animals; Binding Sites; Biochemistry; Cell Line; Cell lines; Chickens; Collagen - metabolism; Electrophoresis, Polyacrylamide Gel; Embryo, Mammalian; Fibroblasts; Fibroblasts - analysis; Fibronectins - isolation & purification; Hamsters; Humans; Kidney; Kidney cells; Mice; Molecular Weight; Peptide Fragments - analysis; Rats; Transformed cell line; Trypsin; Tumor cell line; Tumors; Urinary Bladder Neoplasms
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.1.28

Tryptic peptide mapping of fibronectin from various species indicates that (i) there exists a remarkable similarity between methionine-labeled tryptic peptides of fibronectins isolated from cells of widely separated species such as human, chicken, mouse, hamster, and rat; (ii) fibronectins from normal and transformed or tumor cells of the same species are very similar, if not identical; and (iii) collagen-binding regions of fibronectin from different species share identical major peptides.