Inoculation of tomato plants (Solanum lycopersicum) with growth-promoting Bacillus subtilis retards whitefly Bemisia tabaci development

• Published in: Planta Vol. 231; no. 2; pp. 397 - 410
• Main Authors: Valenzuela-Soto, José Humberto, Estrada-Hernández, María Gloria, Ibarra-Laclette, Enrique, Délano-Frier, John Paul
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Adult insects; Adults; Agriculture; Animals; Bacillus subtilis; Bacillus subtilis - cytology; Bacillus subtilis - physiology; Biological and medical sciences; Biomedical and Life Sciences; Biosynthesis; Cluster Analysis; Colony Count, Microbial; Ecology; Forestry; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Hemiptera - growth & development; Host-Pathogen Interactions; Infestation; Inoculation; Life Sciences; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Lycopersicon esculentum - microbiology; Lycopersicon esculentum - parasitology; Mutation - genetics; Oligonucleotide Array Sequence Analysis; Original Article; Pest resistance; Plant growth; Plant growth promoting rhizobacteria; Plant Proteins - metabolism; Plant Sciences; Plants; Population Dynamics; Proteinase inhibitors; Pupae; Reproducibility of Results; Rhizosphere; RNA; Tomatoes; Salicylic acid; Growth promoting rhizobacteria; Induced systemic response; Jasmonic acid; Phenyl propanoids; Retardation; Bacillus subtilis; Insecta; Plant biology; Systemic; Bacillaceae; Inoculation; Pest; Bacillales; Homoptera; Dicotyledones; Angiospermae; Lycopersicon esculentum; Development; Bacteria; Plant growth substance; Solanaceae; Terpenoid; Induced systemic responses; Response; Rhizobacteria; Vegetable crop; Arthropoda; Bemisia tabaci; Spermatophyta; Aleyrodidae; Invertebrata
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-009-1061-9

Root inoculation of tomato (Solanum lycopersicum) plants with a Bacillus subtilis strain BEB-DN (BsDN) isolated from the rhizosphere of cultivated potato plants was able to promote growth and to generate an induced systemic resistance (ISR) response against virus-free Bemisia tabaci. Growth promotion was evident 3 weeks after inoculation. No changes in oviposition density, preference and nymphal number in the early stages of B. tabaci development were observed between BsDN-treated plants and control plants inoculated with a non-growth promoting Bs strain (PY-79), growth medium or water. However, a long-term ISR response was manifested by a significantly reduced number of B. tabaci pupae developing into adults in BsDN-treated plants. The observed resistance response appeared to be a combination of jasmonic acid (JA) dependent and JA-independent responses, since the BsDN-related retardation effect on B. tabaci development was still effective in the highly susceptible spr2 tomato mutants with an impaired capacity for JA biosynthesis. A screening of 244 genes, 169 of which were previously obtained from subtractive-suppressive-hybridization libraries generated from B. tabaci-infested plants suggested that the BsDN JA-dependent ISR depended on an anti-nutritive effect produced by the simultaneous expression of genes coding principally for proteases and proteinase inhibitors, whereas the JA-independent ISR observed in the spr2 background curiously involved the up-regulation of several photosynthetic genes, key components of the phenyl-propanoid and terpenoid biosynthetic pathways and of the Hsp90 chaperonin, which probably mediated pest resistance response(s), in addition to the down-regulation of pathogenesis and hypersensitive response genes.