Kidney proteome responses in the teleost fish Paralichthys olivaceus indicate a putative immune response against Streptococcus parauberis

• Published in: Journal of proteomics Vol. 75; no. 17; pp. 5166 - 5175
• Main Authors: Cha, In-Seok, Kwon, Joseph, Park, Seong-Hwa, Nho, Seong-Won, Jang, Ho-Bin, Park, Seong-Bin, del Castillo, Carmelo S., Hikima, Jun-ichi, Aoki, Takashi, Jung, Tae-Sung
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 18-09-2012; Elsevier
• Subjects: Adaptive Immunity - physiology; Animals; Bacterial infection; Biological and medical sciences; calreticulin; cathepsins; cell division; Cluster Analysis; complement; Diverse techniques; fish; Fish Diseases - immunology; Fish Diseases - metabolism; Flounder - immunology; Flounder - metabolism; Fundamental and applied biological sciences. Psychology; gene expression regulation; glycoproteins; heat shock proteins; immune response; Immune System - chemistry; Immune System - metabolism; Kidney; Kidney - chemistry; Kidney - immunology; Kidney - metabolism; kidneys; Label-free; lysozyme; Marine; Models, Biological; Molecular and cellular biology; Paralichthys olivaceus; protein synthesis; proteome; Proteome - analysis; Proteome - metabolism; Proteomics; Streptococcal Infections - immunology; Streptococcal Infections - metabolism; Streptococcus - immunology; Streptococcus parauberis; tandem mass spectrometry; Teleost fish; Teleostei; Tissue Distribution; ABC; DTT; Teleost fish; MRM; MHC; LMP7; PLGS; PICR; IgM; Kidney; SRM; emPAI; CDC48; GLUT-1; TLR; ISG; Paralichthys olivaceus; AAA ATPase; PBS; APCs; TSB; TSA; HSP; MS; EST; IAA; SILAC; ER; ICAT; Bacterial infection; SDS-PAGE; CAN; TCA; UPLC; NAG; LC; Proteomics; iTRAQ; IPA; NAM; Label-free; Immune response; Proteome; Infection; Streptococcaceae; Vertebrata; Streptococcus; Pisces; Bacteriosis; Bacteria; Micrococcales
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2012.05.046

The proteomic response to bacterial infection in a teleost fish (Paralichthys olivaceus) infected with Streptococcus parauberis was analyzed using label-free protein quantitation coupled with LC–MSE tandem mass spectrometry. A total of 82 proteins from whole kidney, a major lymphoid organ in this fish, were found to be differentially expressed between healthy and diseased fish analyzed 6, 24, 72 and 120h post-infection. Among the differentially expressed proteins, those involved in mediating immune responses (e.g., heat shock proteins, cathepsins, goose-type lysozyme and complement components) were most significantly up-regulated by infection. In addition, cell division cycle 48 (CDC48) and calreticulin, which are associated with cellular recovery and glycoprotein synthesis, were up-regulated in the universal protein group, whereas the other proteins in that group were down-regulated. There was continuous activation of expression of immune-associated proteins during infection, but there was also loss of expression of proteins not involved in immune function. We expect that our findings regarding immune response at the protein level would offer new insight into the systemic response to bacterial infection of a major immune organ in teleost fish.
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► The first comprehensive protein kinetics in teleost fish is described. ► Key proteins in the kidney during the pathogenesis of streptococcosis are presented. ► CDC48 and calreticulin are shown to be pivotal proteins in the cellular repair process. ► The significance of lysozymes is shown in the present analysis.