A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase

• Published in: Current opinion in microbiology Vol. 7; no. 2; pp. 115 - 119
• Main Authors: Starai, VJ, Takahashi, H, Boeke, JD, Escalante-Semerena, JC
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2004
• Subjects: Acetate-CoA Ligase - metabolism; Amino Acid Sequence; Molecular Sequence Data; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Salmonella enterica - genetics; Salmonella enterica - metabolism; Sirtuins; Sirtuins - genetics; Sirtuins - metabolism; Transcription, Genetic - physiology; Acs; NAD; Sir2; NADH; AMP; CoA; OAADPr
• ISSN: 1369-5274; 1879-0364
• DOI: 10.1016/j.mib.2004.02.005

The silent information regulator protein (Sir2) and its homologs (collectively known as sirtuins) are NAD
+-dependent deacetylase enzymes involved in chromosome stability, gene silencing and cell aging in eukaryotes and archaea. The discovery that sirtuin-dependent protein deacetylation is a NAD
+-consuming reaction established a link with the energy generation systems of the cell. This link to metabolism was recently extended to the post-translational control of the activity of short-chain fatty acyl-coenzyme A (adenosine monophosphate-forming) synthetases in bacteria and yeast. The crystal structure of the Sir protein complexed with a peptide of a protein substrate provided insights into how sirtuins interact with their protein substrates.