Lid hinge region of Penicillium expansum lipase affects enzyme activity and interfacial activation

•Sites displaying the highest B factors in the lid were selected for mutagenesis.•Mutants showed properties remarkably different from those of wild-type PEL.•Mutation shifted the chain-length specificity.•One mutant lacked interfacial activation.•We provide a new clue for selecting critical amino ac...

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Bibliographic Details

Published in:	Process biochemistry (1991) Vol. 50; no. 8; pp. 1218 - 1223
Main Authors:	Tang, Lianghua, Su, Min, Yan, Junzhe, Xie, Sheng, Zhang, Wenhuang
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 01-08-2015
Subjects:	Activation Amino acids Biochemistry Hinges Lid hinge Lipase Mutagenesis Penicillium Penicillium expansum Residues Saturation Penicillium expansum Lid hinge Mutagenesis Lipase
Online Access:	Get full text
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