Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus

• Published in: Molecular & cellular proteomics Vol. 8; no. 1; pp. 19 - 31
• Main Authors: Lee, Joohyun, Feng, Jian, Campbell, Kimberly B., Scheffler, Brian E., Garrett, Wesley M., Thibivilliers, Sandra, Stacey, Gary, Naiman, Daniel Q., Tucker, Mark L., Pastor-Corrales, M.A., Cooper, Bret
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-2009; American Society for Biochemistry and Molecular Biology
• Subjects: Basidiomycota - pathogenicity; Basidiomycota - physiology; beans; disease resistance; Gene Expression Regulation, Plant; host-pathogen relationships; Immunity, Innate; leaves; liquid chromatography; mass spectrometry; Models, Biological; Phaseolus - genetics; Phaseolus - metabolism; Phaseolus - microbiology; Phaseolus vulgaris; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Leaves - genetics; Plant Leaves - microbiology; plant proteins; Plant Proteins - analysis; Plant Proteins - genetics; proteome; Proteome - analysis; Proteomics; races; rust diseases; Uromyces appendiculatus; Virulence
• ISSN: 1535-9476; 1535-9484; 1535-9484
• DOI: 10.1074/mcp.M800156-MCP200

Plants appear to have two types of active defenses, a broad-spectrum basal system and a system controlled by R-genes providing stronger resistance to some pathogens that break the basal defense. However, it is unknown if the systems are separate entities. Therefore, we analyzed proteins from leaves of the dry bean crop plant Phaseolus vulgaris using a high-throughput liquid chromatography tandem mass spectrometry method. By statistically comparing the amounts of proteins detected in a single plant variety that is susceptible or resistant to infection, depending on the strains of a rust fungus introduced, we defined basal and R-gene-mediated plant defenses at the proteomic level. The data reveal that some basal defense proteins are potential regulators of a strong defense weakened by the fungus and that the R-gene modulates proteins similar to those in the basal system. The results satisfy a new model whereby R-genes are part of the basal system and repair disabled defenses to reinstate strong resistance.