Optimisation and validation of a liquid chromatography–tandem mass spectrometry method for folates in rice

An LC–MS/MS method has been developed and validated for the analysis of six monoglutamate folates in rice. Accurate determination of folates in rice seeds required the optimisation of an extraction procedure encompassing homogenisation, heat treatment, tri-enzyme treatment (α-amylase, protease and d...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 1215; no. 1; pp. 125 - 132
Main Authors:	De Brouwer, Veerle, Storozhenko, Sergei, Van De Steene, Jet C., Wille, Sarah M.R., Stove, Christophe P., Van Der Straeten, Dominique, Lambert, Willy E.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 26-12-2008 Amsterdam; New York: Elsevier Elsevier
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences Calibration Cereal and baking product industries Chemical constitution Chromatography, High Pressure Liquid - methods Economic plant physiology Folates folic acid Folic Acid - analysis food analysis Food industries Freshwater Fundamental and applied biological sciences. Psychology LC–MS/MS liquid chromatography mass spectrometry monoglutamate folates Oryza - chemistry Oryza sativa Reproducibility of Results rice Rice analysis Sensitivity and Specificity tandem mass spectrometry Tandem Mass Spectrometry - methods Tri-enzyme treatment vitamin content Folates LC–MS/MS Rice analysis Tri-enzyme treatment Validation Tandem mass spectrometry Elution Gradient Chemical analysis Coupled method Vitamin Pteridine derivatives HPLC chromatography Folic acid Folate Optimization α-Aminoacid LC-MS/MS Reversed phase chromatography Cereal Quantitative analysis Rice
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