Inter- and intra-specific cuticle variation between amphimictic and parthenogenetic species of root-knot nematode ( Meloidogyne spp.) as revealed by a bacterial parasite ( Pasteuria penetrans)

• Published in: International journal for parasitology Vol. 38; no. 7; pp. 851 - 859
• Main Authors: Davies, K.G., Rowe, J.A., Williamson, V.M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-06-2008; [Oxford; New York]: Elsevier Science; Elsevier Science
• Subjects: amphimixis; animal cuticle; Animals; Bacteria; Bacterial Adhesion; Biological and medical sciences; endospores; Female; Fundamental and applied biological sciences. Psychology; Gram-Positive Endospore-Forming Bacteria - physiology; Host-Parasite Interactions; host-parasite relationships; hyperparasitism; Immunity; interspecific variation; intraspecific variation; Invertebrates; Life cycle. Host-agent relationship. Pathogenesis; line differences; Male; Meloidogyne; Meloidogyne hapla; Meloidogyne incognita; Mode of reproduction; Nemathelminthia. Plathelmintha; Nematoda; Parasites - physiology; Parasitology - methods; Parthenogenesis; Pasteuria penetrans; Pathogenesis; Plant parasitic nematode; Plant Roots - parasitology; Protozoa; Reproduction - physiology; root-knot nematodes; sexual reproduction; species differences; Species Specificity; spore adhesion; spore attachment; Spores, Bacterial - physiology; strain differences; Surface coat; Tylenchoidea - anatomy & histology; Tylenchoidea - immunology; Tylenchoidea - parasitology; Plant parasitic nematode; Pathogenesis; Immunity; Surface coat; Mode of reproduction; Parthenogenesis; Meloidogyne; Parasite; Pasteuria penetrans; Cuticle; Reproduction; Pest; Phytophagous; Helmintha; Bacteria; Actinomycetes; Nemathelminthia; Invertebrata; Nematoda
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/j.ijpara.2007.11.007

Specific host–parasite interactions exist between species and strains of plant parasitic root-knot nematodes and the Gram-positive bacterial hyperparasite
Pasteuria penetrans. This bacterium produces endospores that adhere to the cuticle of migrating juveniles, germinate and colonise the developing female within roots. Endospore attachment of
P. penetrans populations to second-stage juveniles of the root-knot nematode species
Meloidogyne incognita and
Meloidogyne hapla showed there were interactive differences between bacterial populations and nematode species. Infected females of
M. incognita produced a few progeny which were used to establish two nematode lines from single infective juveniles encumbered with either three or 26 endospores. Single juvenile descent lines of each nematode species were produced to test whether cuticle variation was greater within
M. hapla lines that reproduce by facultative meiotic parthenogenesis than within lines of
M. incognita, which reproduces by obligate parthenogenesis. Assays revealed variability between broods of individual females derived from single second-stage juvenile descent lines of both
M. incognita and
M. hapla suggesting that progeny derived from a single individual can differ in spore adhesion in both sexual and asexual nematode species. These results suggest that special mechanisms that produced these functional differences in the cuticle surface may have evolved in both sexually and asexually reproducing nematodes as a strategy to circumvent infection by this specialised hyperparasite.