Optimum conditions for transformed Panax ginseng hairy roots in flask culture

• Published in: Applied biochemistry and biotechnology Vol. 98; no. 1-9; pp. 1129 - 1139
• Main Authors: Jeong, G.T, Park, D.H, Ryu, H.W, Lee, W.T, Park, K, Kang, C.H, Hwang, B, Woo, J.C
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Heidelberg Springer 2002; Springer Nature B.V
• Subjects: Agrobacterium rhizogenes; Biological and medical sciences; Biomass; Biotechnology; branching; carbon; Carbon sources; Cell Culture Techniques - methods; Cells, Cultured; Complex media; Culture Media; Eukaryotic cell cultures; Fructose; Fundamental and applied biological sciences. Psychology; Genetics; Ginseng; Glucose; Hairy root; Herbs; Inoculum; Kinetics; Lactose - metabolism; Methods. Procedures. Technologies; Miscellaneous; Monosaccharides - metabolism; nitrogen; Panax - growth & development; Panax - microbiology; Panax ginseng; phosphates; Phosphates - metabolism; Plant cells and fungal cells; Plant Roots - growth & development; Plant Roots - microbiology; Polysaccharides; Polysaccharides - metabolism; Rhizobium - metabolism; Rhizobium rhizogenes; Roots; salt concentration; Saponins; Saponins - isolation & purification; Saponins - metabolism; Sucrose; Sucrose - metabolism; Culture medium; Hairy root; Vegetals; Genetic transformation; Tissue culture; Panax ginseng; Optimization; Dicotyledones; Angiospermae; Bacteria; Spermatophyta; Rhizobiaceae; Agrobacterium rhizogenes; Araliaceae
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:98-100:1-9:1129

Panax ginseng hairy roots were transformed by Agrobacterium rhizogenes KTCT 2744. They showed an active branching pattern and fast growth in hormone-free medium, and good growth at 23 degrees C, pH 5.8, 1/2 MS medium, and 3% sucrose. Sucrose provided the highest growth among seven carbon sources tested. Six complex media were also tested. In the combined sugar study, hairy roots grew better on sucrose without glucose or fructose than with glucose or fructose. In the 1/2 MS basal medium, 30 mM in nitrogen and 0.62 mM phosphate salt concentration was the optimum. The growth ratio was maximal at an inoculum size of 0.4% (w/v). Crude saponin and polysaccharide levels were also measured.