Mass production of genetically modified Aedes aegypti for field releases in Brazil

• Published in: Journal of visualized experiments no. 83; p. e3579
• Main Authors: Carvalho, Danilo O, Nimmo, Derric, Naish, Neil, McKemey, Andrew R, Gray, Pam, Wilke, André B B, Marrelli, Mauro T, Virginio, Jair F, Alphey, Luke, Capurro, Margareth L
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 04-01-2014
• Subjects: Aedes - genetics; Aedes - growth & development; Animals; Animals, Genetically Modified; Basic Protocol; Brazil; Female; Genes, Dominant; Genes, Lethal; Male; Pest Control, Biological - methods; Reproduction - physiology; Brazil
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/3579

New techniques and methods are being sought to try to win the battle against mosquitoes. Recent advances in molecular techniques have led to the development of new and innovative methods of mosquito control based around the Sterile Insect Technique (SIT)(1-3). A control method known as RIDL (Release of Insects carrying a Dominant Lethal)(4), is based around SIT, but uses genetic methods to remove the need for radiation-sterilization(5-8). A RIDL strain of Ae. aegypti was successfully tested in the field in Grand Cayman(9,10); further field use is planned or in progress in other countries around the world. Mass rearing of insects has been established in several insect species and to levels of billions a week. However, in mosquitoes, rearing has generally been performed on a much smaller scale, with most large scale rearing being performed in the 1970s and 80s. For a RIDL program it is desirable to release as few females as possible as they bite and transmit disease. In a mass rearing program there are several stages to produce the males to be released: egg production, rearing eggs until pupation, and then sorting males from females before release. These males are then used for a RIDL control program, released as either pupae or adults(11,12). To suppress a mosquito population using RIDL a large number of high quality male adults need to be reared(13,14). The following describes the methods for the mass rearing of OX513A, a RIDL strain of Ae. aegypti (8), for release and covers the techniques required for the production of eggs and mass rearing RIDL males for a control program.