Matricellular Protein Periostin Contributes to Hepatic Inflammation and Fibrosis

• Published in: The American journal of pathology Vol. 185; no. 3; pp. 786 - 797
• Main Authors: Huang, Yangmei, Liu, Weiping, Xiao, Hongjun, Maitikabili, Alaiyi, Lin, Qinghua, Wu, Tiantian, Huang, Zhengjie, Liu, Fan, Luo, Qi, Ouyang, Gaoliang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-03-2015
• Subjects: Animals; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Collagen - metabolism; Hepatic Stellate Cells - metabolism; Hepatic Stellate Cells - pathology; Hepatitis - metabolism; Hepatitis - pathology; Humans; Inflammation - metabolism; Inflammation - pathology; Liver - metabolism; Liver - pathology; Liver Cirrhosis - metabolism; Liver Cirrhosis - pathology; Mice; Mice, Knockout; Pathology; Transforming Growth Factor beta - metabolism
• ISSN: 0002-9440; 1525-2191
• DOI: 10.1016/j.ajpath.2014.11.002

Periostin actively contributes to tissue injury, fibrosis, atherosclerosis, and inflammatory diseases; however, its role in hepatic fibrosis is unclear. Herein, we revealed that periostin expression was significantly up-regulated in carbon tetrachloride– and bile duct ligation–induced mice with acute and chronic liver fibrosis. Deficiency in periostin abrogated the development of liver fibrosis in mice. Carbon tetrachloride treatment significantly increased α-smooth muscle actin, fibronectin, and collagen I levels in wild-type mice, which were unaffected in periostin-knockout mice. Periostin-deficient mice showed a significantly reduced area of collagen deposition and decreased levels of serum alanine aminotransferase and aspartate aminotransferase compared with wild-type mice after 2 weeks of carbon tetrachloride administration. Chemokine ligand 2, IL-6, IL-1β, tumor necrosis factor-α, and tissue inhibitor of metalloproteinases 1 mRNA levels were significantly lower in periostin-deficient mice than in wild-type mice after carbon tetrachloride treatment. Periostin colocalized with hepatic stellate cell–derived collagen I and α-smooth muscle actin in mouse acute and chronic fibrotic liver tissues. Transforming growth factor (TGF)-β1 markedly induced periostin expression in primary mouse hepatic stellate cells. Periostin-deficient mice showed significantly lower levels of TGF-β1 and TGF-β2 compared with wild-type mice after carbon tetrachloride treatment. High levels of periostin in patients with acute or chronic hepatitis correlated with TGF-β1 and TGF-β2 expression in serum from patients with hepatitis. Data indicate that periostin is a novel mediator of hepatic fibrosis development.