Glucose uptake stimulatory effect of 4-hydroxypipecolic acid by increased GLUT 4 translocation in skeletal muscle cells

• Published in: Bioorganic & medicinal chemistry letters Vol. 22; no. 17; pp. 5648 - 5651
• Main Authors: Naresh, G., Jaiswal, N., Sukanya, P., Srivastava, A.K., Tamrakar, A.K., Narender, T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01-09-2012; Elsevier
• Subjects: 4-Hydroxypipecolic acid; Amino acid; Animals; Antihyperglycemic activity; Biological and medical sciences; Biological Transport - drug effects; Cell Line; Cell surface; chemistry; Drug development; drugs; General pharmacology; Glucose; Glucose - metabolism; Glucose transport; Glucose Transporter Type 4 - metabolism; glucose transporters; glycemic effect; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Insulin - metabolism; Medical sciences; Medicinal plants; Muscle, Skeletal - cytology; Muscle, Skeletal - metabolism; myocytes; Peganum - chemistry; Peganum harmala; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pipecolic Acids - isolation & purification; Pipecolic Acids - pharmacology; program planning; Rats; Seeds; Skeletal muscle; Translocation; Zygophyllaceae; India; Amino acid; Antihyperglycemic activity; 4-Hydroxypipecolic acid; Glucose transport; Peganum harmala; Translocation; Aldose; Pharmacognosy; Glucose; Striated muscle; In vitro; Biological activity; Uptake; Medicinal plant; Hypoglycemic agent; Pipecolic acid derivatives; Zygophyllaceae; Dicotyledones; Aminoacid; Angiospermae; Plant origin; Spermatophyta; Transport
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2012.06.101

Peganum harmala Linn, commonly known as ‘harmal’ belonging to the family Zygophyllaceae, is one of the most important medicinal plants of India. In continuation of our drug development program on Indian medicinal plants we discovered antihyperglycemic activity in 4-hydroxypipecolic acid (4-HPA), isolated from the seed of P. harmala. Effect of 4-HPA on glucose uptake and glucose transporter-4 (GLUT-4) translocation was investigated in L6 skeletal muscle cell lines. Treatment with 4-HPA stimulated both glucose uptake and GLUT4 translocation from intracellular to cell surface in skeletal muscle cells in a concentration-dependent manner, which might be leading to antihyperglycemic effect.