Early complement activation increases in the brain in some aged normal subjects

• Published in: Neurobiology of aging Vol. 25; no. 8; pp. 1001 - 1007
• Main Authors: Loeffler, David A, Camp, Dianne M, Schonberger, Michael B, Singer, Daniel J, LeWitt, Peter A
• Format: Journal Article
• Language: English
• Published: London Elsevier Inc 01-09-2004; Elsevier Science
• Subjects: Adult; Aged; Aged, 80 and over; Aging; Aging - immunology; Aging - metabolism; Alzheimer Disease - immunology; Alzheimer Disease - metabolism; Alzheimer Disease - physiopathology; Alzheimer’s disease; Biological and medical sciences; Brain; Brain - immunology; Brain - metabolism; Brain - physiopathology; Complement activation; Complement C3b - metabolism; Complement C4 - metabolism; Complement C4b; Complement System Proteins - immunology; Complement System Proteins - metabolism; Development. Senescence. Regeneration. Transplantation; ELISA; Encephalitis - immunology; Encephalitis - metabolism; Encephalitis - physiopathology; Entorhinal Cortex - immunology; Entorhinal Cortex - metabolism; Entorhinal Cortex - physiopathology; Fundamental and applied biological sciences. Psychology; Hippocampus - immunology; Hippocampus - metabolism; Hippocampus - physiopathology; Humans; Inflammation; Middle Aged; Peptide Fragments - metabolism; Reference Values; Up-Regulation - physiology; Vertebrates: nervous system and sense organs; Aging; Brain; Complement activation; Inflammation; Alzheimer’s disease; ELISA; Human; Senescence; Central nervous system; Activation; Complement; ELISA assay; Encephalon
• ISSN: 0197-4580; 1558-1497
• DOI: 10.1016/j.neurobiolaging.2003.11.003

Complement activation is increased in Alzheimer’s disease (AD) and may contribute to the development and progression of this disorder. To compare early complement activation between normal and AD brain specimens, C4d and iC3b concentrations were measured in hippocampus, entorhinal cortex, temporal cortex, parietal cortex, and cerebellum from aged normal and AD subjects (
n=10–14 for both), and in hippocampus and entorhinal cortex from younger normal subjects (
n=5–6). C4d and iC3b levels increased 2.3- to 4.6-fold in AD versus aged normal specimens (all
P<0.05), with lowest concentrations of these activation proteins generally in cerebellum. No significant differences were present between aged and younger normal C4d and iC3b levels in hippocampus or entorhinal cortex. However, the concentrations of these proteins were markedly increased in several aged normal specimens. Normal subject age was moderately associated with both C4d (
r=0.49) and iC3b (
r=0.53) concentrations in the hippocampus. Increased brain complement activation in some elderly individuals may promote the subsequent development of AD.